Universität zu Köln

Forsdahl, Guro, Erceg, Damir, Geisendorfer, Thomas, Turkalj, Mirjana, Plavec, Davor ORCID: 0000-0003-2020-8119, Thevis, Mario, Tretzel, Laura and Gmeiner, Guenter (2015). Detection of testosterone esters in blood. Drug Test. Anal., 7 (11-12). S. 983 - 990. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

Injections of synthetic esters of testosterone are among the most common forms of testosterone application. In doping control, the detection of an intact ester of testosterone in blood gives unequivocal proof of the administration of exogenous testosterone. The aim of the current project was to investigate the detection window for injected testosterone esters as a mixed substance preparation and as a single substance preparation in serum and plasma. Furthermore, the suitability of different types of blood collection devices was evaluated. Collection tubes with stabilizing additives, as well as non-stabilized serum separation tubes, were tested. A clinical study with six participants was carried out, comprising a single intramuscular injection of either 1000mg testosterone undecanoate (Nebido((R))) or a mixture of 30mg testosterone propionate, 60mg testosterone phenylpropionate, 60mg testosterone isocaproate, and 100mg testosterone decanoate (Sustanon((R))). Blood was collected throughout a testing period of 60days. The applied analytical method for blood analysis included liquid-liquid extraction and preparation of oxime derivatives, prior to TLX-sample clean-up and liquid chromatography-tandem mass spectrometry (LC-MS/MS) detection. All investigated testosterone esters could be detected in post-administration blood samples. The detection time depended on the type of ester administered. Furthermore, results from the study show that measured blood concentrations of especially short-chained testosterone esters are influenced by the type of blood collection device applied. The testosterone ester detection window, however, was comparable. Copyright (c) 2015 John Wiley & Sons, Ltd.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Forsdahl, Guro UNSPECIFIED UNSPECIFIED UNSPECIFIED Erceg, Damir UNSPECIFIED UNSPECIFIED UNSPECIFIED Geisendorfer, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Turkalj, Mirjana UNSPECIFIED UNSPECIFIED UNSPECIFIED Plavec, Davor UNSPECIFIED orcid.org/0000-0003-2020-8119 UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Tretzel, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Gmeiner, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-387637
DOI:	10.1002/dta.1914
Journal or Publication Title:	Drug Test. Anal.
Volume:	7
Number:	11-12
Page Range:	S. 983 - 990
Date:	2015
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1942-7611
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MASS-SPECTROMETRY ANALYSIS; HUMAN HAIR; DOPING CONTROL; HUMAN-PLASMA; ANABOLIC-STEROIDS; URINARY STEROIDS; ISOTOPE; UNDECANOATE; NANDROLONE; RATIOS Multiple languages Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38763