Universität zu Köln

Boisson-Dernier, Aurelien ORCID: 0000-0002-9790-3710, Franck, Christina Maria, Lituiev, Dmytro S. and Grossniklaus, Ueli ORCID: 0000-0002-0522-8974 (2015). Receptor-like cytoplasmic kinase MARIS functions downstream of CrRLK1L-dependent signaling during tip growth. Proc. Natl. Acad. Sci. U. S. A., 112 (39). S. 12211 - 12217. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

Growing plant cells need to rigorously coordinate external signals with internal processes. For instance, the maintenance of cell wall (CW) integrity requires the coordination of CW sensing with CW remodeling and biosynthesis to avoid growth arrest or integrity loss. Despite the involvement of receptor-like kinases (RLKs) of the Catharanthus roseus RLK1-like (CrRLK1L) subfamily and the reactive oxygen species-producing NADPH oxidases, it remains largely unknown how this coordination is achieved. ANXUR1 (ANX1) and ANX2, two redundant members of the CrRLK1L subfamily, are required for tip growth of the pollen tube (PT), and their closest homolog, FERONIA, controls root-hair tip growth. Previously, we showed that ANX1 overexpression mildly inhibits PT growth by over-secretion of CW material, whereas pollen tubes of anx1 anx2 double mutants burst spontaneously after germination. Here, we report the identification of suppressor mutants with improved fertility caused by the rescue of anx1 anx2 pollen tube bursting. Mapping of one these mutants revealed an R240C nonsynonymous substitution in the activation loop of a receptor-like cytoplasmic kinase (RLCK), which we named MARIS (MRI). We show that MRI is a plasma membrane-localized member of the RLCK-VIII subfamily and is preferentially expressed in both PTs and root hairs. Interestingly, mri-knockout mutants display spontaneous PT and root-hair bursting. Moreover, expression of the MRIR240C mutant, but not its wild-type form, partially rescues the bursting phenotypes of anx1 anx2 PTs and fer root hairs but strongly inhibits wild-type tip growth. Thus, our findings identify a novel positive component of the CrRLK1L-dependent signaling cascade that coordinates CW integrity and tip growth.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Boisson-Dernier, Aurelien UNSPECIFIED orcid.org/0000-0002-9790-3710 UNSPECIFIED Franck, Christina Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Lituiev, Dmytro S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Grossniklaus, Ueli UNSPECIFIED orcid.org/0000-0002-0522-8974 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-392629
DOI:	10.1073/pnas.1512375112
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	112
Number:	39
Page Range:	S. 12211 - 12217
Date:	2015
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLLEN-TUBE RECEPTION; PROTEIN-KINASE; ARABIDOPSIS-THALIANA; PLANT IMMUNITY; AGC KINASES; FERONIA; WALL; PATHWAY; ACID; OXI1 Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/39262