Universität zu Köln

Swaminathan, Karthic ORCID: 0000-0002-0981-7549, Stumpf, Maria, Mueller, Rolf, Horn, Anna-Carolin, Schmidbauer, Julia, Eichinger, Ludwig ORCID: 0000-0003-1594-6117, Mueller-Taubenberger, Annette, Faix, Jan and Noegel, Angelika A. (2015). Coronin7 regulates WASP and SCAR through CRIB mediated interaction with Rac proteins. Sci Rep, 5. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Coronin7 (CRN7) stabilizes F-actin and is a regulator of processes associated with the actin cytoskeleton. Its loss leads to defects in phagocytosis, motility and development. It harbors a CRIB (Cdc42- and Rac-interactive binding) domain in each of its WD repeat domains which bind to Rac GTPases preferably in their GDP-loaded forms. Expression of wild type CRN7 in CRN7 deficient cells rescued these defects, whereas proteins with mutations in the CRIB motifs which were associated with altered Rac binding were effective to varying degrees. The presence of one functional CRIB was sufficient to reestablish phagocytosis, cell motility and development. Furthermore, by molecular modeling and mutational analysis we identified the contact regions between CRN7 and the GTPases. We also identified WASP, SCAR and PAKa as downstream effectors in phagocytosis, development and cell surface adhesion, respectively, since ectopic expression rescued these functions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Swaminathan, Karthic UNSPECIFIED orcid.org/0000-0002-0981-7549 UNSPECIFIED Stumpf, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Rolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Horn, Anna-Carolin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidbauer, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Eichinger, Ludwig UNSPECIFIED orcid.org/0000-0003-1594-6117 UNSPECIFIED Mueller-Taubenberger, Annette UNSPECIFIED UNSPECIFIED UNSPECIFIED Faix, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-392659
DOI:	10.1038/srep14437
Journal or Publication Title:	Sci Rep
Volume:	5
Date:	2015
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DICTYOSTELIUM-DISCOIDEUM; ACTIN-BINDING; F-ACTIN; CELL MOTILITY; MONOCLONAL-ANTIBODIES; CYTOKINESIS; CHEMOTAXIS; CYTOSKELETON; TRAFFICKING; HOMOLOG Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39265