Taylor, Susan H., Yeung, Ching-Yan Chloe ORCID: 0000-0002-7076-8109, Kalson, Nicholas S., Lu, Yinhui, Zigrino, Paola ORCID: 0000-0002-7470-0064, Starborg, Tobias, Warwood, Stacey, Holmes, David F., Canty-Laird, Elizabeth G., Mauch, Cornelia and Kadler, Karl E. (2015). Matrix metalloproteinase 14 is required for fibrous tissue expansion. eLife, 4. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD. ISSN 2050-084X

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Abstract

Type I collagen-containing fibrils are major structural components of the extracellular matrix of vertebrate tissues, especially tendon, but how they are formed is not fully understood. MMP14 is a potent pericellular collagenase that can cleave type I collagen in vitro. In this study, we show that tendon development is arrested in Scleraxis-Cre::Mmp14 lox/lox mice that are unable to release collagen fibrils from plasma membrane fibripositors. In contrast to its role in collagen turnover in adult tissue, MMP14 promotes embryonic tissue formation by releasing collagen fibrils from the cell surface. Notably, the tendons grow to normal size and collagen fibril release from fibripositors occurs in Col-r/r mice that have a mutated collagen-I that is uncleavable by MMPs. Furthermore, fibronectin ( not collagen-I) accumulates in the tendons of Mmp14-null mice. We propose a model for cell-regulated collagen fibril assembly during tendon development in which MMP14 cleaves a molecular bridge tethering collagen fibrils to the plasma membrane of fibripositors.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Taylor, Susan H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yeung, Ching-Yan ChloeUNSPECIFIEDorcid.org/0000-0002-7076-8109UNSPECIFIED
Kalson, Nicholas S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lu, YinhuiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zigrino, PaolaUNSPECIFIEDorcid.org/0000-0002-7470-0064UNSPECIFIED
Starborg, TobiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Warwood, StaceyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Holmes, David F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Canty-Laird, Elizabeth G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mauch, CorneliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kadler, Karl E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-392774
DOI: 10.7554/eLife.09345
Journal or Publication Title: eLife
Volume: 4
Date: 2015
Publisher: ELIFE SCIENCES PUBLICATIONS LTD
Place of Publication: CAMBRIDGE
ISSN: 2050-084X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GELATINASE-A MMP-2; EXTRACELLULAR-MATRIX; PERIODONTAL-LIGAMENT; COLLAGEN FIBRILS; PERIOSTIN; MICE; IDENTIFICATION; FIBRONECTIN; SITE; LOCALIZATIONMultiple languages
BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/39277

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