Universität zu Köln

Kann, Martin, Ettou, Sandrine, Jung, Youngsook L., Lenz, Maximilian O., Taglienti, Mary E., Park, Peter J., Schermer, Bernhard ORCID: 0000-0002-5194-9000, Benzing, Thomas and Kreidberg, Jordan A. (2015). Genome-Wide Analysis of Wilms' Tumor 1-Controlled Gene Expression in Podocytes Reveals Key Regulatory Mechanisms. J. Am. Soc. Nephrol., 26 (9). S. 2097 - 2105. WASHINGTON: AMER SOC NEPHROLOGY. ISSN 1533-3450

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Abstract

The transcription factor Wilms' tumor suppressor 1 (WT1) is key to podocyte development and viability; however, WT1 transcriptional networks in podocytes remain elusive. We provide a comprehensive analysis of the genome-wide WT1 transcriptional network in podocytes in vivo using chromatin immunoprecipitation followed by sequencing (ChIPseq) and RNA sequencing techniques. Our data show a specific role for WT1 in regulating the podocyte-specific transcriptome through binding to both promoters and enhancers of target genes. Furthermore, we inferred a podocyte transcription factor network consisting of WT1, LMX1B, TCF21, Fox-class and TEAD family transcription factors, and MAFB that uses tissue-specific enhancers to control podocyte gene expression. In addition to previously described WT1-dependent target genes, ChIPseq identified novel WT1-dependent signaling systems. These targets included components of the Hippo signaling system, underscoring the power of genome-wide transcriptional-network analyses. Together, our data elucidate a comprehensive gene regulatory network in podocytes suggesting that WT1 gene regulatory function and podocyte cell-type specification can best be understood in the context of transcription factor-regulatory element network interplay.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kann, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Ettou, Sandrine UNSPECIFIED UNSPECIFIED UNSPECIFIED Jung, Youngsook L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lenz, Maximilian O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Taglienti, Mary E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Park, Peter J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schermer, Bernhard UNSPECIFIED orcid.org/0000-0002-5194-9000 UNSPECIFIED Benzing, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Kreidberg, Jordan A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-395658
DOI:	10.1681/ASN.2014090940
Journal or Publication Title:	J. Am. Soc. Nephrol.
Volume:	26
Number:	9
Page Range:	S. 2097 - 2105
Date:	2015
Publisher:	AMER SOC NEPHROLOGY
Place of Publication:	WASHINGTON
ISSN:	1533-3450
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NEPHROTIC SYNDROME; WT1; LMX1B; DIFFERENTIATION; TRANSCRIPTION; SUPPRESSOR; FILTRATION; PROMOTER; YAP/TAZ; GROWTH Multiple languages Urology & Nephrology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39565