Universität zu Köln

Kuhlmann, Nora, Petrov, Dimitar P., Henrich, Alexander W., Lindner, Steffen N., Wendisch, Volker F. ORCID: 0000-0003-3473-0012 and Seibold, Gerd M. (2015). Transcription of malP is subject to phosphotransferase system-dependent regulation in Corynebacterium glutamicum. Microbiology-(UK), 161. S. 1830 - 1844. LONDON: MICROBIOLOGY SOC. ISSN 1465-2080

Full text not available from this repository.

Abstract

The Gram-positive Corynebacterium glutamicum co-metabolizes most carbon sources such as the phosphotransferase system (PTS) sugar glucose and the non-PTS sugar maltose. Maltose is taken up via the ABC-transporter MusEFGK(2)I, and is further metabolized to glucose phosphate by amylomaltase MalQ, maltodextrin phosphorylase MalP, glucokinase Glk and phosophoglucomutase Pgm. Surprisingly, growth of C. glutamicum strains lacking the general PTS components El or HPr was strongly impaired on the non-PTS sugar maltose. Complementation experiments showed that a functional PTS phosphorelay is required for optimal growth of C. glutamicum on maltose, implying its involvement in the control of maltose metabolism and/or uptake. To identify the target of this PTS-dependent control, transport measurements with C-14-labelled maltose, Northern blot analyses and enzyme assays were performed. The activities of the maltose transporter and enzymes MalQ, Pgm and GIK were not decreased in PTS-deficient C. glutamicum strains, which was corroborated by comparable transcript amounts of musE, musk and musG, as well as of ma/Q, in C. glutamicum Delta ptsH and WT. By contrast, MalP activity was significantly reduced and only residual amounts of malP transcripts were detected in C. glutamicum Delta ptsH when compared to WT. Promoter activity assays with the malP promoter in C. glutamicum Delta ptsH and WT confirmed that malP transcription is reduced in the PTS-deficient strain. Taken together, we show here for what is to the best of our knowledge the first time a regulatory function of the PTS in C. glutamicum and identify malP transcription as its target.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kuhlmann, Nora UNSPECIFIED UNSPECIFIED UNSPECIFIED Petrov, Dimitar P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Henrich, Alexander W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lindner, Steffen N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wendisch, Volker F. UNSPECIFIED orcid.org/0000-0003-3473-0012 UNSPECIFIED Seibold, Gerd M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-395737
DOI:	10.1099/mic.0.000134
Journal or Publication Title:	Microbiology-(UK)
Volume:	161
Page Range:	S. 1830 - 1844
Date:	2015
Publisher:	MICROBIOLOGY SOC
Place of Publication:	LONDON
ISSN:	1465-2080
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CARBON CATABOLITE REPRESSION; ESCHERICHIA-COLI; BACILLUS-SUBTILIS; INDUCER EXCLUSION; ENZYME-I; CATALYZED PHOSPHORYLATION; MALTOSE TRANSPORTER; GENE-EXPRESSION; SUGAR-TRANSPORT; BACTERIAL PHOSPHOENOLPYRUVATE Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39573