Universität zu Köln

Rodrigues, Robim M., Sachinidis, Agapios, De Boe, Veerle, Rogiers, Vera ORCID: 0000-0003-0635-7740, Vanhaecke, Tamara ORCID: 0000-0002-6685-7299 and De Kock, Joery ORCID: 0000-0002-4078-4896 (2015). Identification of potential biomarkers of hepatitis B-induced acute liver failure using hepatic cells derived from human skin precursors. Toxicol. Vitro, 29 (6). S. 1231 - 1240. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 0887-2333

Full text not available from this repository.

Abstract

Besides their role in the elucidation of pathogenic processes of medical and pharmacological nature, biomarkers can also be used to document specific toxicological events. Hepatic cells generated from human skin-derived precursors (hSKP-HPC) were previously shown to be a promising in vitro tool for the evaluation of drug-induced hepatotoxicity. In this study, their capacity to identify potential liver-specific biomarkers at the gene expression level was investigated with particular emphasis on acute liver failure (ALF). To this end, a set of potential ALF-specific biomarkers was established using clinically relevant liver samples obtained from patients suffering from hepatitis B-associated ALF. Subsequently, this data was compared to data obtained from primary human hepatocyte cultures and hSKP-HPC, both exposed to the ALF-inducing reference compound acetaminophen. It was found that both in vitro systems revealed a set of molecules that was previously identified in the ALF liver samples. Yet, only a limited number of molecules was common between both in vitro systems and the ALF liver samples. Each of the in vitro systems could be used independently to identify potential toxicity biomarkers related to ALF. It seems therefore more appropriate to combine primary human hepatocyte cultures with complementary in vitro models to efficiently screen out potential hepatotoxic compounds. (C) 2014 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rodrigues, Robim M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED De Boe, Veerle UNSPECIFIED UNSPECIFIED UNSPECIFIED Rogiers, Vera UNSPECIFIED orcid.org/0000-0003-0635-7740 UNSPECIFIED Vanhaecke, Tamara UNSPECIFIED orcid.org/0000-0002-6685-7299 UNSPECIFIED De Kock, Joery UNSPECIFIED orcid.org/0000-0002-4078-4896 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-396079
DOI:	10.1016/j.tiv.2014.10.012
Journal or Publication Title:	Toxicol. Vitro
Volume:	29
Number:	6
Page Range:	S. 1231 - 1240
Date:	2015
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	0887-2333
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language STEM-CELLS; TOXICITY; INJURY; TRANSPLANTATION; HEPATOTOXICITY; HEPATOCYTES; PROTEOMICS; EXPRESSION; MODELS Multiple languages Toxicology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39607