Universität zu Köln

Frerigmann, Henning, Glawischnig, Erich ORCID: 0000-0001-9280-5065 and Gigolashvili, Tamara ORCID: 0000-0002-0416-4796 (2015). The role of MYB34, MYB51 and MYB122 in the regulation of camalexin biosynthesis in Arabidopsis thaliana. Front. Plant Sci., 6. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

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Abstract

The phytoalexin camalexin and indolic glucosinolates share not only a common evolutionary origin and a tightly interconnected biosynthetic pathway, but regulatory proteins controlling the shared enzymatic steps are also modulated by the same R2R3-MYB transcription factors. The indolic phytoalexin camalexin is a crucial defense metabolite in the model plant Arabidopsis. indolic phytoalexins and glucosinolates appear to have a common evolutionary origin and are interconnected on the biosynthetic level: a key intermediate in the biosynthesis of camalexin, indole-3-acetaldoxime (IA0x), is also required for the biosynthesis of indolic glucosinolates and is under tight control by the transcription factors MYB34, MYB51, and MYB122. The abundance of camalexin was strongly reduced in myb34/51 and myb51/122 double and in triple myb mutant, suggesting that these transcription factors are important in camalexin biosynthesis. Furthermore, expression of MYB51 and MYB122 was significantly increased by biotic and abiotic camalexin-inducing agents. Feeding of the triple myb34/51/122 mutant with IA0x or indole-3-acetonitrile largely restored camalexin biosynthesis. Conversely, tryptophan could not complement the low camalexin phenotype of this mutant, which supports a role for the three MYB factors in camalexin biosynthesis upstream of IA0x. Consistently expression of the camalexin biosynthesis genes CYP71815/PAD3 and CYP71A13 was not negatively affected in the triple myb mutant and the MYBs could not activate pCYP71815::uidA expression in trans-activation assays with cultured Arabidopsis cells. In conclusion, this study reveals the importance of MYB factors regulating the generation of IA0x as precursor of camalexin.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Frerigmann, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Glawischnig, Erich UNSPECIFIED orcid.org/0000-0001-9280-5065 UNSPECIFIED Gigolashvili, Tamara UNSPECIFIED orcid.org/0000-0002-0416-4796 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-396216
DOI:	10.3389/fpls.2015.00654
Journal or Publication Title:	Front. Plant Sci.
Volume:	6
Date:	2015
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-462X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INDOLIC GLUCOSINOLATE BIOSYNTHESIS; TRANSCRIPTION FACTOR; AUXIN HOMEOSTASIS; CYTOCHROME-P450 MONOOXYGENASE; PHYTOALEXIN BIOSYNTHESIS; GENE-EXPRESSION; INDOLE-3-ACETALDOXIME; TRYPTOPHAN; INFECTION; CATALYZES Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39621