Universität zu Köln

Di Cristanziano, Veronica, Timmen-Wego, Monika, Luebke, Nadine, Kaiser, Rolf, Pfister, Herbert, Di Cave, David, Berrilli, Federica, Kabore, Yolande and D'Alfonso, Rossella (2015). Application of Luminex Gastrointestinal Pathogen Panel to human stool samples from Cote d'Ivoire. J. Infect. Dev. Ctries., 9 (8). S. 884 - 890. TRAMANIGLIO: J INFECTION DEVELOPING COUNTRIES. ISSN 1972-2680

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Abstract

Introduction: Gastrointestinal infections caused by viruses, bacteria, and parasites are endemic in most developing countries due to inadequate provision of safe water supplies, sanitation, and hygiene. To investigate the enteric pathogens infecting people living in Cote d'Ivoire, the Luminex Gastrointestinal Pathogen Panel (xTAG GPP) assay was used to analyze 34 human fecal samples. This study represents the first application of this technology to samples from a sub-Saharan African country. Methodology: Thirty-four stool samples from asymptomatic and symptomatic patients, 1-15 years of age, were analyzed by xTAG GPP. The Luminex assay represents a qualitative bead-based multiplexed molecular diagnostic test able to identify concurrently 15 enteric pathogens, including bacteria, viruses, and parasites. Results: Overall, 22 out of 34 (64.7%) fecal specimens were detected to be positive by xTAG GPP. Sixteen were from asymptomatic subjects, and 10 patients (45.4%) showed co-infections. G. duodenalis was detected in 15 patients, in both mono-and co-infections, representing the most frequent pathogen, followed by enterotoxigenic Escherichia coli (ETEC) LT/ST. Four norovirus isolates were also detected and assigned to genogroups I and II. Conclusions: Considering the burden of enteric infections in developing countries, particularly among children, and the high rate of coinfections in asymptomatic subjects, this study shows the need for diagnostic tools such as xTAG GPP to improve diagnosis and treatment of these infections in endemic areas.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Di Cristanziano, Veronica UNSPECIFIED UNSPECIFIED UNSPECIFIED Timmen-Wego, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Luebke, Nadine UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaiser, Rolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfister, Herbert UNSPECIFIED UNSPECIFIED UNSPECIFIED Di Cave, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Berrilli, Federica UNSPECIFIED UNSPECIFIED UNSPECIFIED Kabore, Yolande UNSPECIFIED UNSPECIFIED UNSPECIFIED D'Alfonso, Rossella UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-397138
DOI:	10.3855/jidc.6460
Journal or Publication Title:	J. Infect. Dev. Ctries.
Volume:	9
Number:	8
Page Range:	S. 884 - 890
Date:	2015
Publisher:	J INFECTION DEVELOPING COUNTRIES
Place of Publication:	TRAMANIGLIO
ISSN:	1972-2680
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GIARDIA-DUODENALIS; MOLECULAR CHARACTERIZATION; ENVIRONMENTAL ENTEROPATHY; CHILDHOOD DIARRHEA; CHILDREN; PREVALENCE; EPIDEMIOLOGY; BACTERIAL; ETIOLOGY; ANIMALS Multiple languages Infectious Diseases Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39713