Universität zu Köln

Loeschcke, Anita and Thies, Stephan ORCID: 0000-0003-4240-9149 (2015). Pseudomonas putida-a versatile host for the production of natural products. Appl. Microbiol. Biotechnol., 99 (15). S. 6197 - 6215. NEW YORK: SPRINGER. ISSN 1432-0614

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Abstract

The biosynthesis of natural products by heterologous expression of biosynthetic pathways in amenable production strains enables biotechnological access to a variety of valuable compounds by conversion of renewable resources. Pseudomonas putida has emerged as a microbial laboratory work horse, with elaborated techniques for cultivation and genetic manipulation available. Beyond that, this bacterium offers several particular advantages with regard to natural product biosynthesis, notably a versatile intrinsic metabolism with diverse enzymatic capacities as well as an outstanding tolerance to xenobiotics. Therefore, it has been applied for recombinant biosynthesis of several valuable natural products. This review provides an overview of applications of P. putida as a host organism for the recombinant biosynthesis of such natural products, including rhamnolipids, terpenoids, polyketides and non-ribosomal peptides, and other amino acid-derived compounds. The focus is on de novo natural product synthesis from intrinsic building blocks by means of heterologous gene expression and strain engineering. Finally, the future potential of the bacterium as a chassis organism for synthetic microbiology is pointed out.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Loeschcke, Anita UNSPECIFIED UNSPECIFIED UNSPECIFIED Thies, Stephan UNSPECIFIED orcid.org/0000-0003-4240-9149 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-397947
DOI:	10.1007/s00253-015-6745-4
Journal or Publication Title:	Appl. Microbiol. Biotechnol.
Volume:	99
Number:	15
Page Range:	S. 6197 - 6215
Date:	2015
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-0614
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COMPLETE GENOME SEQUENCE; FIELD-GROWN WHEAT; RESISTANT STAPHYLOCOCCUS-AUREUS; BIOSYNTHETIC GENE-CLUSTER; HETEROLOGOUS EXPRESSION; ESCHERICHIA-COLI; RHAMNOLIPID PRODUCTION; SYNTHETIC BIOLOGY; POLYKETIDE BIOSYNTHESIS; LYSOBACTER-LACTAMGENUS Multiple languages Biotechnology & Applied Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39794