Universität zu Köln

Krzisch, Marine, Temprana, Silvio G., Mongiat, Lucas A., Armida, Jan, Schmutz, Valentin ORCID: 0000-0002-0935-6121, Virtanen, Mari A., Kocher-Braissant, Jacqueline, Kraftsik, Rudolf, Vutskits, Laszlo, Conzelmann, Karl-Klaus ORCID: 0000-0002-8614-3656, Bergami, Matteo ORCID: 0000-0001-5525-5025, Gage, Fred H., Schinder, Alejandro F. and Toni, Nicolas ORCID: 0000-0001-5585-268X (2015). Pre-existing astrocytes form functional perisynaptic processes on neurons generated in the adult hippocampus. Brain Struct. Funct., 220 (4). S. 2027 - 2043. HEIDELBERG: SPRINGER HEIDELBERG. ISSN 1863-2661

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Abstract

The adult dentate gyrus produces new neurons that morphologically and functionally integrate into the hippocampal network. In the adult brain, most excitatory synapses are ensheathed by astrocytic perisynaptic processes that regulate synaptic structure and function. However, these processes are formed during embryonic or early postnatal development and it is unknown whether astrocytes can also ensheathe synapses of neurons born during adulthood and, if so, whether they play a role in their synaptic transmission. Here, we used a combination of serial-section immuno-electron microscopy, confocal microscopy, and electrophysiology to examine the formation of perisynaptic processes on adult-born neurons. We found that the afferent and efferent synapses of newborn neurons are ensheathed by astrocytic processes, irrespective of the age of the neurons or the size of their synapses. The quantification of gliogenesis and the distribution of astrocytic processes on synapses formed by adult-born neurons suggest that the majority of these processes are recruited from pre-existing astrocytes. Furthermore, the inhibition of astrocytic glutamate re-uptake significantly reduced postsynaptic currents and increased paired-pulse facilitation in adult-born neurons, suggesting that perisynaptic processes modulate synaptic transmission on these cells. Finally, some processes were found intercalated between newly formed dendritic spines and potential presynaptic partners, suggesting that they may also play a structural role in the connectivity of new spines. Together, these results indicate that pre-existing astrocytes remodel their processes to ensheathe synapses of adult-born neurons and participate to the functional and structural integration of these cells into the hippocampal network.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Krzisch, Marine UNSPECIFIED UNSPECIFIED UNSPECIFIED Temprana, Silvio G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mongiat, Lucas A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Armida, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmutz, Valentin UNSPECIFIED orcid.org/0000-0002-0935-6121 UNSPECIFIED Virtanen, Mari A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kocher-Braissant, Jacqueline UNSPECIFIED UNSPECIFIED UNSPECIFIED Kraftsik, Rudolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Vutskits, Laszlo UNSPECIFIED UNSPECIFIED UNSPECIFIED Conzelmann, Karl-Klaus UNSPECIFIED orcid.org/0000-0002-8614-3656 UNSPECIFIED Bergami, Matteo UNSPECIFIED orcid.org/0000-0001-5525-5025 UNSPECIFIED Gage, Fred H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schinder, Alejandro F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Toni, Nicolas UNSPECIFIED orcid.org/0000-0001-5585-268X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-399721
DOI:	10.1007/s00429-014-0768-y
Journal or Publication Title:	Brain Struct. Funct.
Volume:	220
Number:	4
Page Range:	S. 2027 - 2043
Date:	2015
Publisher:	SPRINGER HEIDELBERG
Place of Publication:	HEIDELBERG
ISSN:	1863-2661
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LONG-TERM POTENTIATION; ENHANCED SYNAPTIC PLASTICITY; DENTATE GYRUS; RAT HIPPOCAMPUS; 3-DIMENSIONAL RELATIONSHIPS; EXCITATORY SYNAPSES; CNS SYNAPTOGENESIS; PATTERN SEPARATION; DENDRITIC SPINES; GLUTAMATE UPTAKE Multiple languages Anatomy & Morphology; Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39972