Gehlsen, Uta ORCID: 0000-0001-9057-9199, Szaszak, Marta, Gebert, Andreas, Koop, Norbert, Huettmann, Gereon and Steven, Philipp (2015). Non-Invasive Multi-Dimensional Two-Photon Microscopy enables optical fingerprinting (TPOF) of immune cells. J. Biophotonics, 8 (6). S. 466 - 480. WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1864-0648

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Abstract

Mucosal surfaces are constantly exposed to pathogens and show high immunological activity. In a broad variety of ocular surface disorders inflammation is common, but underlying mechanisms are often not fully understood. However, the main clinical problem is that inflammatory processes are difficult to characterize and quantify due to the impossibility of repeated tissue probing of the delicate ocular surface. Therefore non-invasive optical methods are thought to have the potential for intravital investigation of ocular surface inflammation. This study demonstrates the general potential of two-photon microscopy to non-invasively detect and discriminate key players of inflammation in the ocular surface by using intrinsic fluorescence-based features without the necessity of tissue probing or the use of dyes. The use of wavelength dependent measurements of fluorescence lifetime, in addition to autofluorescence intensity enables a functional differentiation of isolated immune cells in vitro at excitation wavelengths between 710 to 830 nm. Mixed cell cultures and first in vivo results indicate the use of excitation wavelength of 710 to 750 nm for further experiments and future use in patients.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Gehlsen, UtaUNSPECIFIEDorcid.org/0000-0001-9057-9199UNSPECIFIED
Szaszak, MartaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gebert, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koop, NorbertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huettmann, GereonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Steven, PhilippUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-402525
DOI: 10.1002/jbio.201400036
Journal or Publication Title: J. Biophotonics
Volume: 8
Number: 6
Page Range: S. 466 - 480
Date: 2015
Publisher: WILEY-V C H VERLAG GMBH
Place of Publication: WEINHEIM
ISSN: 1864-0648
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
IN-VIVO; IMAGING REVEALS; 2ND-HARMONIC GENERATION; IMPRESSION CYTOLOGY; DENDRITIC CELLS; LYMPHOID-TISSUE; FLOW-CYTOMETRY; AUTOFLUORESCENCE; MULTIPHOTON; MACROPHAGESMultiple languages
Biochemical Research Methods; Biophysics; OpticsMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/40252

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