Universität zu Köln

Johnson, Sarah, Weddell, Sarah, Godbert, Sonya, Freundl, Guenter, Roos, Judith and Gnoth, Christian (2015). Development of the first urinary reproductive hormone ranges referenced to independently determined ovulation day. Clin. Chem. Lab. Med., 53 (7). S. 1099 - 1109. BERLIN: WALTER DE GRUYTER GMBH. ISSN 1437-4331

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Abstract

Background: Urinary hormone level analysis provides valuable fertility status information; however, previous studies have not referenced levels to the ovulation day, or have used outdated methods. This study aimed to produce reproductive hormone ranges referenced to ovulation day determined by ultrasound. Methods: Women aged 18-40 years (no reported infertility) collected daily urine samples for one complete menstrual cycle. Urinary luteinising hormone (LH), estrone-3- glucuronide (E3G, an estradiol metabolite), follicle stimulating hormone (FSH) and pregnanediol-3-glucuronide (P3G, a progesterone metabolite) were measured using previously validated assays. Volunteers underwent trans-vaginal ultrasound every 2 days until the dominant ovarian follicle size reached 16 mm, when daily scans were performed until ovulation was observed. Data were analysed to create hormone ranges referenced to the day of objective ovulation as determined by ultrasound. Results: In 40 volunteers, mean age 28.9 years, urinary LH surge always preceded ovulation with a mean of 0.81 days; thus LH is an excellent assay-independent predictor of ovulation. The timing of peak LH was assay-dependent and could be post-ovulatory; therefore should no longer be used to predict/determine ovulation. Urinary P3G rose from baseline after ovulation in all volunteers, peaking a median of 7.5 days following ovulation. Median urinary peak E3G and FSH levels occurred 0.5 days prior to ovulation. A persistent rise in urinary E3G was observed from approximately 3 days pre-until 5 days post-ovulation. Conclusions: This study provides reproductive hormone ranges referenced to the actual day of ovulation as determined by ultrasound, to facilitate examination of menstrual cycle endocrinology.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Johnson, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Weddell, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Godbert, Sonya UNSPECIFIED UNSPECIFIED UNSPECIFIED Freundl, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED Roos, Judith UNSPECIFIED UNSPECIFIED UNSPECIFIED Gnoth, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-402873
DOI:	10.1515/cclm-2014-1087
Journal or Publication Title:	Clin. Chem. Lab. Med.
Volume:	53
Number:	7
Page Range:	S. 1099 - 1109
Date:	2015
Publisher:	WALTER DE GRUYTER GMBH
Place of Publication:	BERLIN
ISSN:	1437-4331
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLYCYSTIC-OVARY-SYNDROME; LUTEINIZING-HORMONE; MENSTRUAL-CYCLE; UNITED-STATES; WOMEN; FERTILITY; PREVALENCE; CONCEPTION; ANOVULATION; VARIABILITY Multiple languages Medical Laboratory Technology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40287