Universität zu Köln

Matthaei, Mario, Gillessen, Johannes, Muether, Philipp S., Hoerster, Robert, Bachmann, Bjoern O., Hueber, Arno ORCID: 0000-0003-2157-126X, Cursiefen, Claus and Heindl, Ludwig M. (2015). Epithelial-Mesenchymal Transition (EMT)-Related Cytokines in the Aqueous Humor of Phakic and Pseudophakic Fuchs' Dystrophy Eyes. Invest. Ophthalmol. Vis. Sci., 56 (4). S. 2749 - 2755. ROCKVILLE: ASSOC RESEARCH VISION OPHTHALMOLOGY INC. ISSN 1552-5783

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Abstract

PURPOSE. The present study aimed to analyze the expression of epithelial-mesenchymal transition (EMT)-related cytokines in the aqueous humor of phakic and pseudophakic Fuchs' endothelial corneal dystrophy (FECD) eyes and their correlation to FECD severity. METHODS. Aqueous humor samples from phakic FECD eyes (FECDph, n = 9), from pseudophakic FECD eyes more than 1 year after cataract surgery (FECDpsph, n = 13), and from cataract controls without FECD (Control(cat), n = 28) were obtained during Descemet membrane endothelial keratoplasty (DMEK) or cataract surgery. Expression of EMT-related cytokines (TGF-beta 1, TGF-beta 2, TGF-beta 3, MCP-1, BFGF, TNF-alpha, IL-1 beta) was measured using multiplex bead assay. Corneal central-to-peripheral thickness ratio at 3.5 mm from the center (CPTR3.5) was determined as an objective metric for FECD severity before surgery by slit-scanning pachymetry. RESULTS. Pseudophakic FECD eyes showed significantly elevated expression compared with Controlcat and FECDph eyes for TGF-beta 1 (P < 0.001, respectively), for TGF-beta 2 (P < 0.05, respectively), and MCP-1 (P < 0.001, respectively). Levels of TGF-b1 (r = 0.6116, P < 0.05) and MCP-1 (r = 0.5934, P < 0.05) were positively correlated with CPTR3.5. No differences in EMT-associated protein levels were detected comparing FECDph eyes and Controlcat eyes. CONCLUSIONS. Simultaneous elevation of TGF-beta 1, TGF-beta 2, and MCP-1 concentrations in FECDpsph eyes confirms that cataract surgery leads to long-term alterations of the intraocular microenvironment. Positive correlation of increased aqueous TGF-beta 1 and MCP-1 levels with CPTR3.5 in pseudophakic FECD eyes suggests that changed cytokine levels may be involved in corneal decompensation after cataract surgery. Unchanged aqueous humor levels of EMT-related proteins analyzed in phakic FECD patients indicate that there is no primary role of these aqueous cytokines in FECD pathogenesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Matthaei, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Gillessen, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Muether, Philipp S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoerster, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Bachmann, Bjoern O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hueber, Arno UNSPECIFIED orcid.org/0000-0003-2157-126X UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, Ludwig M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-404189
DOI:	10.1167/iovs.15-16395
Journal or Publication Title:	Invest. Ophthalmol. Vis. Sci.
Volume:	56
Number:	4
Page Range:	S. 2749 - 2755
Date:	2015
Publisher:	ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Place of Publication:	ROCKVILLE
ISSN:	1552-5783
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOTHELIAL CORNEAL-DYSTROPHY; OPEN-ANGLE GLAUCOMA; DESCEMETS-MEMBRANE; CATARACT-SURGERY; GROWTH-FACTOR; INTRAOCULAR CONCENTRATIONS; MACULAR DEGENERATION; KERATOPLASTY; POSTERIOR; AGE Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40418