Universität zu Köln

Tempfer, Herbert ORCID: 0000-0003-4043-4033, Kaser-Eichberger, Alexandra, Korntner, Stefanie ORCID: 0000-0002-5937-9938, Lehner, Christine, Kunkel, Nadja, Traweger, Andreas ORCID: 0000-0002-0220-4766, Trost, Andrea, Strohmaier, Clemens, Bogner, Barbara, Runge, Christian, Bruckner, Daniela, Krefft, Karolina, Heindl, Ludwig M., Reitsamer, Herbert A. and Schroedl, Falk (2015). Presence of lymphatics in a rat tendon lesion model. Histochem. Cell Biol., 143 (4). S. 411 - 420. NEW YORK: SPRINGER. ISSN 1432-119X

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Abstract

Tendons lack sufficient blood supply and represent a bradytroph tissue with prolonged healing time under pathological conditions. While the role of lymphatics in wound/defect healing in tissues with regular blood supply is well investigated, its involvement in tendon defects is not clear. We here try to identify the role of the lymphatic system in a tendon lesion model with morphological methods. A rat Achilles tendon lesion model (n = 5) was created via surgical intervention. Two weeks after surgery, animals were killed and lesioned site removed and prepared for polarization microscopy (picrosirius red) and immunohistochemistry using the lymphatic markers PROX1, VEGFR3, CCL21, LYVE-1, PDPN, and the vascular marker CD31. Additionally, DAPI was applied. Untreated tendons served as controls, confocal laser-scanning microscopy was used for documentation. At the lesion site, polarization microscopy revealed a structural reintegration while immunohistochemistry detected band-like profiles immunoreactive for PDPN, VEGFR3, CCL21, LYVE1, and CD31, surrounding DAPI-positive nuclei. PROX1-positive nuclei were detected within the lesion forming lines and opposed to each other. These PROX1-positive nuclei were surrounded by LYVE-1- or VEGFR3-positive surfaces. Few CD31-positve profiles contained PROX1-positive nuclei, while the majority of CD31-positive profiles lacked PROX1-positive nuclei. VEGFR3-, PDPN-, and LYVE-1-positive profiles were numerous within the lesion site, but absent in control tissue. Within 2 weeks, a structural rearrangement takes place in this lesion model, with dense lymphatic supply. The role of lymphatics in tendon wound healing is unclear, and proposed model represents a good possibility to study healing dynamics and lymphangiogenesis in a tissue almost completely lacking lymphatics in physiological conditions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tempfer, Herbert UNSPECIFIED orcid.org/0000-0003-4043-4033 UNSPECIFIED Kaser-Eichberger, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Korntner, Stefanie UNSPECIFIED orcid.org/0000-0002-5937-9938 UNSPECIFIED Lehner, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Kunkel, Nadja UNSPECIFIED UNSPECIFIED UNSPECIFIED Traweger, Andreas UNSPECIFIED orcid.org/0000-0002-0220-4766 UNSPECIFIED Trost, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Strohmaier, Clemens UNSPECIFIED UNSPECIFIED UNSPECIFIED Bogner, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Runge, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Bruckner, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Krefft, Karolina UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, Ludwig M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Reitsamer, Herbert A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroedl, Falk UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-408582
DOI:	10.1007/s00418-014-1287-x
Journal or Publication Title:	Histochem. Cell Biol.
Volume:	143
Number:	4
Page Range:	S. 411 - 420
Date:	2015
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-119X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACHILLES-TENDON; CONNECTIVE-TISSUE; LYMPHANGIOGENESIS; ANGIOGENESIS; INFLAMMATION; VASCULATURE; ENDOTHELIUM; EXPRESSION; DISEASE; BIOLOGY Multiple languages Cell Biology; Microscopy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40858