Universität zu Köln

Keuters, Meike Hedwig ORCID: 0000-0003-4216-1024, Aswendt, Markus ORCID: 0000-0003-1423-0934, Tennstaedt, Annette, Wiedermann, Dirk, Pikhovych, Anton, Rotthues, Steffen, Fink, Gereon Rudolf, Schroeter, Michael, Hoehn, Mathias and Rueger, Maria Adele (2015). Transcranial direct current stimulation promotes the mobility of engrafted NSCs in the rat brain. NMR Biomed., 28 (2). S. 231 - 240. HOBOKEN: WILEY-BLACKWELL. ISSN 1099-1492

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Abstract

Transcranial direct current stimulation (tDCS) is used in numerous clinical studies and considered an effective and versatile add-on therapy in neurorehabilitation. To date, however, the underlying neurobiological mechanisms remain elusive. In a rat model of tDCS, we recently observed a polarity-dependent accumulation of endogenous neural stem cells (NSCs) in the stimulated cortex. Based upon these findings, we hypothesized that tDCS may exert a direct migratory effect on endogenous NSCs towards the stimulated cortex. Using noninvasive imaging, we here investigated whether tDCS may also cause a directed migration of engrafted NSCs. Murine NSCs were labeled with superparamagnetic particles of iron oxide (SPIOs) and implanted into rat striatum and corpus callosum. MRI was performed (i) immediately after implantation and (ii) after 10 tDCS sessions of anodal or cathodal polarity. Sham-stimulated rats served as control. Imaging results were validated ex vivo using immunohistochemistry. Overall migratory activity of NSCs almost doubled after anodal tDCS. However, no directed migration within the electric field (i.e. towards or away from the electrode) could be observed. Rather, an undirected outward migration from the center of the graft was detected. Xenograft transplantation induced a neuroinflammatory response that was significantly enhanced following cathodal tDCS. This inflammatory response did not impact negatively on the survival of implanted NSCs. Data suggest that anodal tDCS increases the undirected migratory activity of implanted NSCs. Since the electric field did not guide implanted NSCs over large distances, previously observed polarity-dependent accumulation of endogenous NSCs in the cortex might have originated from local proliferation. Results enhance our understanding of the neurobiological mechanisms underlying tDCS, and may thereby help to develop a targeted and sustainable application of tDCS in clinical practice. Copyright (c) 2014 John Wiley & Sons, Ltd.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Keuters, Meike Hedwig UNSPECIFIED orcid.org/0000-0003-4216-1024 UNSPECIFIED Aswendt, Markus UNSPECIFIED orcid.org/0000-0003-1423-0934 UNSPECIFIED Tennstaedt, Annette UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiedermann, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED Pikhovych, Anton UNSPECIFIED UNSPECIFIED UNSPECIFIED Rotthues, Steffen UNSPECIFIED UNSPECIFIED UNSPECIFIED Fink, Gereon Rudolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeter, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoehn, Mathias UNSPECIFIED UNSPECIFIED UNSPECIFIED Rueger, Maria Adele UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-414121
DOI:	10.1002/nbm.3244
Journal or Publication Title:	NMR Biomed.
Volume:	28
Number:	2
Page Range:	S. 231 - 240
Date:	2015
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1099-1492
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NEURAL STEM-CELLS; NONINVASIVE CORTICAL STIMULATION; IN-VIVO; ELECTRIC-FIELD; MIGRATION; STROKE; SURVIVAL; MOTOR; CORTEX; ROBUST Multiple languages Biophysics; Radiology, Nuclear Medicine & Medical Imaging; Spectroscopy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41412