Universität zu Köln

Wang, Jian, Masika, Jacob, Zhou, Jianxia, Wang, Jun, Zhu, Minjie, Luo, Hongyan, Hu, Xinwu, Zhang, Liangpin, Tang, Ming, Gao, Linlin, Hescheler, Juergen and Liang, Huamin (2015). Traditional Chinese Medicine Baicalin Suppresses mESCs Proliferation through Inhibition of miR-294 Expression. Cell. Physiol. Biochem., 35 (5). S. 1868 - 1877. BASEL: KARGER. ISSN 1421-9778

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Abstract

Background: Traditional Chinese herbal medicines (TCMs) have been widely used against a broad spectrum of biological activities, including influencing the cardiac differentiation from mouse embryonic stem cells (mESCs). However, their effects and mechanisms of action on ESCs proliferation remain to be determined. The present study aimed to determine the effect of three TCMs, baicalin, ginsenoside Rg1, and puerarin, on mESCs proliferation and to elucidate the possible mechanism of their action. Methods: Cell proliferation was examined with a cell proliferation assay Cell Counting Kit-8 (CCK-8), propidium iodide (PI) staining was used to visualize cell cycle. The mRNA expression level of c-myc, c-fos, c-jun, GAPDH and microRNAs were measured by quantitative real time RT-PCR. Results: We found that baicalin 50 mu M suppressed the proliferation of mESCs as observations in more cells in G1 phase and less cells in either S phase or G2/M phase. Moreover, baicalin suppressed the expressions of c-jun and c-fos in mESCs and down-regulated the expression of miR-294. Overexpression of miR-294 in mESCs significantly reversed the effects of baicalin both on mESC proliferation and c-fos/c-jun expression. Conclusions: Baicalin down-regulation of miR-294 may be its key mechanism of action in decreasing mESCs proliferation. Copyright (C) 2015 S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wang, Jian UNSPECIFIED UNSPECIFIED UNSPECIFIED Masika, Jacob UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhou, Jianxia UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Jun UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhu, Minjie UNSPECIFIED UNSPECIFIED UNSPECIFIED Luo, Hongyan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hu, Xinwu UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Liangpin UNSPECIFIED UNSPECIFIED UNSPECIFIED Tang, Ming UNSPECIFIED UNSPECIFIED UNSPECIFIED Gao, Linlin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Liang, Huamin UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-416663
DOI:	10.1159/000373997
Journal or Publication Title:	Cell. Physiol. Biochem.
Volume:	35
Number:	5
Page Range:	S. 1868 - 1877
Date:	2015
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1421-9778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EMBRYONIC STEM-CELLS; SELF-RENEWAL; CYCLE REGULATION; DIFFERENTIATION; CARDIOMYOCYTES; CARCINOMA; APOPTOSIS; PUERARIN; CDK2 Multiple languages Cell Biology; Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41666