Universität zu Köln

von Brandenstein, Melanie, Puetz, Katharina, Schlosser, Monika, Loeser, Heike, Kallinowski, Joachim P., Goedde, Daniel, Buettner, Reinhard, Stoerkel, Stefan and Fries, Jochen W. U. (2015). Vimentin 3, the New Hope, Differentiating RCC versus Oncocytoma. Dis. Markers, 2015. LONDON: HINDAWI LTD. ISSN 1875-8630

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Abstract

Vimentin is currently used to differentiate between malignant renal carcinomas and benign oncocytomas. Recent reports showing Vimentin positive oncocytomas seriously question the validity of this present diagnostic approach. Vimentin 3 is a spliced variant and ends with a unique C-terminal ending after exon 7 which differentiates it from the full length version that has 9 exons. Therefore, the protein size is different; the full length Vimentin version has a protein size of similar to 57 kDa and the truncated version of similar to 47 kDa. We designed an antibody, called Vim3, against the unique C-terminal ending of the Vimentin 3 variant. Using immune histology, immune fluorescence, Western blot, and qRT-PCR analysis, a Vim3 overexpression was detectable exclusively in oncocytoma, making the detection of Vim3 a potential specific marker for benign kidney tumors. This antibody is the first to clearly differentiate benign oncocytoma and the mimicking eosinophilic variants of the RCCs. This differentiation between malignant and benign RCCs is essential for operative planning, follow-up therapy, and patients' survival. In the future the usage of Vimentin antibodies in routine pathology has to be applied with care. Consideration must be given to Vimentin specific binding epitopes otherwise a misdiagnosis of the patients' tumor samples may result.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code von Brandenstein, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Puetz, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlosser, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Loeser, Heike UNSPECIFIED UNSPECIFIED UNSPECIFIED Kallinowski, Joachim P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Goedde, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Buettner, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Stoerkel, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Fries, Jochen W. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-416682
DOI:	10.1155/2015/368534
Journal or Publication Title:	Dis. Markers
Volume:	2015
Date:	2015
Publisher:	HINDAWI LTD
Place of Publication:	LONDON
ISSN:	1875-8630
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RENAL-CELL CARCINOMA; EXPRESSION; DIAGNOSIS; FILAMENTS Multiple languages Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental; Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41668