Universität zu Köln

Baumgartner, Sven, Halbach, Marcel, Krausgrill, Benjamin, Maass, Martina, Srinivasan, Sureshkumar Perumal, Sahito, Raja Ghazanfar Ali, Peinkofer, Gabriel, Nguemo, Filomain, Mueller-Ehmsen, Jochen and Hescheler, Juergen (2015). Electrophysiological and Morphological Maturation of Murine Fetal Cardiomyocytes During Electrical Stimulation In Vitro. J. Cardiovasc. Pharmacol. Ther., 20 (1). S. 104 - 113. THOUSAND OAKS: SAGE PUBLICATIONS INC. ISSN 1940-4034

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Abstract

The aim of this study was to investigate whether continuous electrical stimulation affects electrophysiological properties and cell morphology of fetal cardiomyocytes (FCMs) in culture. Fetal cardiomyocytes at day 14.5 post coitum were harvested from murine hearts and electrically stimulated for 6 days in culture using a custom-made stimulation chamber. Subsequently, action potentials of FCM were recorded with glass microelectrodes. Immunostainings of -Actinin, connexin 43, and vinculin were performed. Expression of ion channel subunits Kcnd2, Slc8a1, Cacna1, Kcnh2, and Kcnb1 was analyzed by quantitative reverse-transcriptase polymerase chain reaction. Action potential duration to 50% and 90% repolarization (APD(50) and APD(90)) of electrically stimulated FCMs were significantly decreased when compared to nonstimulated control FCM. Alignment of cells was significantly higher in stimulated FCM when compared to control FCM. The expression of connexin 43 was significantly increased in stimulated FCM when compared to control FCM. The ratio between cell length and cell width of the stimulated FCM was significantly higher than in control FCM. Kcnh2 and Kcnd2 were upregulated in stimulated FCM when compared to control FCM. Expression of Slc8a1, Cacna1c, and Kcnb1 was not different in stimulated and control FCMs. The decrease in APD(50) observed after electrical stimulation of FCM in vitro corresponds to the electrophysiological maturation of FCM in vivo. Expression levels of ion channels suggest that some important but not all aspects of the complex process of electrophysiological maturation are promoted by electrical stimulation. Parallel alignment, increased connexin 43 expression, and elongation of FCM are signs of a morphological maturation induced by electrical stimulation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Baumgartner, Sven UNSPECIFIED UNSPECIFIED UNSPECIFIED Halbach, Marcel UNSPECIFIED UNSPECIFIED UNSPECIFIED Krausgrill, Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Maass, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Srinivasan, Sureshkumar Perumal UNSPECIFIED UNSPECIFIED UNSPECIFIED Sahito, Raja Ghazanfar Ali UNSPECIFIED UNSPECIFIED UNSPECIFIED Peinkofer, Gabriel UNSPECIFIED UNSPECIFIED UNSPECIFIED Nguemo, Filomain UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller-Ehmsen, Jochen UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-419123
DOI:	10.1177/1074248414536273
Journal or Publication Title:	J. Cardiovasc. Pharmacol. Ther.
Volume:	20
Number:	1
Page Range:	S. 104 - 113
Date:	2015
Publisher:	SAGE PUBLICATIONS INC
Place of Publication:	THOUSAND OAKS
ISSN:	1940-4034
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENGINEERED HEART-TISSUE; FUNCTIONAL EXPRESSION; CARDIAC MYOCYTES; ERG PROTEINS; ION-CHANNEL; MOUSE; RAT; INTEGRATION; MYOCARDIUM Multiple languages Cardiac & Cardiovascular Systems; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41912