Universität zu Köln

Wolf, Michael, Lossdoerfer, Stefan, Kuepper, Katharina and Jaeger, Andreas (2014). Regulation of high mobility group box protein 1 expression following mechanical loading by orthodontic forces in vitro and in vivo. Eur. J. Orthodont., 36 (6). S. 624 - 632. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2210

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Abstract

INTRODUCTION: The chromatin-binding protein high mobility group box protein 1 (HMGB1) can be released into the extracellular milieu by necrotic and damaged cells and functions as an alarmin that is recognized by the innate immune system to initiate and modulate tissue repair. However, little is known about the function of HMGB1 within orthodontic tooth movement. Therefore, it was the aim of the present study to investigate HMGB1 expression by periodontal ligament (PDL) cells challenged by mechanical loading similar to force levels being applied in orthodontic treatment in vitro and to transfer these findings to an in vivo microenvironment in an animal model of tooth movement in rats. In addition, we addressed the question of whether the regulation of HMGB1 expression is modulated in a time-dependent manner in zones of compressive forces. METHODS AND RESULTS: Protein analysis revealed a basal HMGB1 expression in PDL cell cultures and an increased expression when orthodontic forces were applied. In a rat model of tooth movement including 25 animals that received orthodontic tooth movement for 3, 6, 9, and 12 days, HMGB1 protein expression was demonstrated to be regulated in a time-dependent manner as determined by means of immunohistochemistry and histomorphometrical analyses. CONCLUSION: These data indicate a potential role for HMGB1 protein originating from PDL cells in the regulation of orthodontic tooth movement and the periodontal remodelling process by modifying the local microenvironment.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wolf, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Lossdoerfer, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuepper, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Jaeger, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-421743
DOI:	10.1093/ejo/cjt037
Journal or Publication Title:	Eur. J. Orthodont.
Volume:	36
Number:	6
Page Range:	S. 624 - 632
Date:	2014
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2210
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PERIODONTAL-LIGAMENT CELLS; MESENCHYMAL STEM-CELLS; TOOTH ROOT RESORPTION; NECROSIS-FACTOR-ALPHA; INTERMITTENT PTH(1-34); ALVEOLAR BONE; LATE-PHASE; RAT; MOVEMENT; CYTOKINE Multiple languages Dentistry, Oral Surgery & Medicine Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42174