Universität zu Köln

Benstein, Ruben Maximilian ORCID: 0000-0002-5799-7572, Cebi, Zehra, Podola, Bjoern and Melkonian, Michael (2014). Immobilized Growth of the Peridinin-Producing Marine Dinoflagellate Symbiodinium in a Simple Biofilm Photobioreactor. Mar. Biotechnol., 16 (6). S. 621 - 629. NEW YORK: SPRINGER. ISSN 1436-2236

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Abstract

Products from phototrophic dinoflagellates such as toxins or pigments are potentially important for applications in the biomedical sciences, especially in drug development. However, the technical cultivation of these organisms is often problematic due to their sensitivity to hydrodynamic (shear) stress that is a characteristic of suspension-based closed photobioreactors (PBRs). It is thus often thought that most species of dinoflagellates are non-cultivable at a technical scale. Recent advances in the development of biofilm PBRs that rely on immobilization of microalgae may hold potential to circumvent this major technical problem in dinoflagellate cultivation. In the present study, the dinoflagellate Symbiodinium voratum was grown immobilized on a Twin-Layer PBR for isolation of the carotenoid peridinin, an anti-cancerogenic compound. Biomass productivities ranged from 1.0 to 11.0 g m(-2) day(-1) dry matter per vertical growth surface and a maximal biomass yield of 114.5 g m(-2), depending on light intensity, supplementary CO2, and type of substrate (paper or polycarbonate membrane) used. Compared to a suspension culture, the performance of the Twin-Layer PBRs exhibited significantly higher growth rates and maximal biomass yield. In the Twin-Layer PBR a maximal peridinin productivity of 24 mg m(-2) day(-1) was determined at a light intensity of 74 mu mol m(-2) s(-1), although the highest peridinin content per dry weight (1.7 % w/w) was attained at lower light intensities. The results demonstrate that a biofilm-based PBR that minimizes hydrodynamic shear forces is applicable to technical-scale cultivation of dinoflagellates and may foster biotechnological applications of these abundant marine protists.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Benstein, Ruben Maximilian UNSPECIFIED orcid.org/0000-0002-5799-7572 UNSPECIFIED Cebi, Zehra UNSPECIFIED UNSPECIFIED UNSPECIFIED Podola, Bjoern UNSPECIFIED UNSPECIFIED UNSPECIFIED Melkonian, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-422242
DOI:	10.1007/s10126-014-9581-0
Journal or Publication Title:	Mar. Biotechnol.
Volume:	16
Number:	6
Page Range:	S. 621 - 629
Date:	2014
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1436-2236
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RED TIDE DINOFLAGELLATE; ATTACHED CULTIVATION; WASTE-WATER; CAROTENOIDS; BIOMASS; QUANTIFICATION; PIGMENTS; REMOVAL; DINOPHYCEAE; INHIBITION Multiple languages Biotechnology & Applied Microbiology; Marine & Freshwater Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42224