Universität zu Köln

Malinova, Irina, Kunz, Hans-Henning, Alseekh, Saleh, Herbst, Karoline, Fernie, Alisdair R., Gierth, Markus and Fettke, Joerg ORCID: 0000-0003-0137-7611 (2014). Reduction of the Cytosolic Phosphoglucomutase in Arabidopsis Reveals Impact on Plant Growth, Seed and Root Development, and Carbohydrate Partitioning. PLoS One, 9 (11). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Phosphoglucomutase (PGM) catalyses the interconversion of glucose 1-phosphate (G1P) and glucose 6-phosphate (G6P) and exists as plastidial (pPGM) and cytosolic (cPGM) isoforms. The plastidial isoform is essential for transitory starch synthesis in chloroplasts of leaves, whereas the cytosolic counterpart is essential for glucose phosphate partitioning and, therefore, for syntheses of sucrose and cell wall components. In Arabidopsis two cytosolic isoforms (PGM2 and PGM3) exist. Both PGM2 and PGM3 are redundant in function as single mutants reveal only small or no alterations compared to wild type with respect to plant primary metabolism. So far, there are no reports of Arabidopsis plants lacking the entire cPGM or total PGM activity, respectively. Therefore, amiRNA transgenic plants were generated and used for analyses of various parameters such as growth, development, and starch metabolism. The lack of the entire cPGM activity resulted in a strongly reduced growth revealed by decreased rosette fresh weight, shorter roots, and reduced seed production compared to wild type. By contrast content of starch, sucrose, maltose and cell wall components were significantly increased. The lack of both cPGM and pPGM activities in Arabidopsis resulted in dwarf growth, prematurely die off, and inability to develop a functional inflorescence. The combined results are discussed in comparison to potato, the only described mutant with lack of total PGM activity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Malinova, Irina UNSPECIFIED UNSPECIFIED UNSPECIFIED Kunz, Hans-Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Alseekh, Saleh UNSPECIFIED UNSPECIFIED UNSPECIFIED Herbst, Karoline UNSPECIFIED UNSPECIFIED UNSPECIFIED Fernie, Alisdair R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gierth, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Fettke, Joerg UNSPECIFIED orcid.org/0000-0003-0137-7611 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-423216
DOI:	10.1371/journal.pone.0112468
Journal or Publication Title:	PLoS One
Volume:	9
Number:	11
Date:	2014
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language YEAST-DERIVED INVERTASE; STARCH DEGRADATION; CELL-WALL; BIOCHEMICAL-CHARACTERIZATION; ANTISENSE REPRESSION; MALTOSE METABOLISM; THALIANA L; WILD-TYPE; LEAVES; POTATO Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42321