Universität zu Köln

Eberhard, Johanna Maria ORCID: 0000-0002-8367-3870, Hartjen, Philip, Kummer, Silke, Schmidt, Reinhold E., Bockhorn, Maximilian, Lehmann, Clara ORCID: 0000-0002-7042-1578, Balagopal, Ashwin, Hauber, Joachim, van Lunzen, Jan and zur Wiesch, Julian Schulze (2014). CD161(+) MAIT Cells Are Severely Reduced in Peripheral Blood and Lymph Nodes of HIV-Infected Individuals Independently of Disease Progression. PLoS One, 9 (11). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Mucosal-associated invariant T (MAIT) cells are characterized by the combined expression of the semi-invariant T cell receptor (TCR) V alpha 7.2, the lectin receptor CD161, as well as IL-18R, and play an important role in antibacterial host defense of the gut. The current study characterized CD161(+) MAIT and CD161(-)TCRVa7.2(+) T cell subsets within a large cohort of HIV patients with emphasis on patients with slow disease progression and elite controllers. Mononuclear cells from blood and lymph node samples as well as plasma from 63 patients and 26 healthy donors were analyzed by multicolor flow cytometry and ELISA for IL-18, sCD14 and sCD163. Additionally, MAIT cells were analyzed after in vitro stimulation with different cytokines and/or fixed E. coli. Reduced numbers of CD161(+) MAIT cells during HIV infection were detectable in the blood and lymph nodes of all patient groups, including elite controllers. CD161(+) MAIT cell numbers did not recover even after successful antiretroviral treatment. The loss of CD161(+) MAIT cells was correlated with higher levels of MAIT cell activation; an increased frequency of the CD161(-)TCRV alpha 7.2(+)T cell subset in HIV infection was observed. In vitro stimulation of MAIT cells with IL-18 and IL-12, IL-7 and fixed E. coli also resulted in a rapid and additive reduction of the MAIT cell frequency defined by CD161, IL-18R and CCR6. In summary, the irreversible reduction of the CD161(+) MAIT cell subset seems to be an early event in HIV infection that is independent of later stages of the disease. This loss appears to be at least partially due to the distinctive vulnerability of MAIT cells to the pronounced stimulation by microbial products and cytokines during HIV-infection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Eberhard, Johanna Maria UNSPECIFIED orcid.org/0000-0002-8367-3870 UNSPECIFIED Hartjen, Philip UNSPECIFIED UNSPECIFIED UNSPECIFIED Kummer, Silke UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Reinhold E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bockhorn, Maximilian UNSPECIFIED UNSPECIFIED UNSPECIFIED Lehmann, Clara UNSPECIFIED orcid.org/0000-0002-7042-1578 UNSPECIFIED Balagopal, Ashwin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hauber, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED van Lunzen, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED zur Wiesch, Julian Schulze UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-423605
DOI:	10.1371/journal.pone.0111323
Journal or Publication Title:	PLoS One
Volume:	9
Number:	11
Date:	2014
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INVARIANT T-CELLS; IMMUNE ACTIVATION; SOLUBLE CD163; HETEROGENEITY; EXPRESSION; RESPONSES; THERAPY; PATHWAY; ABSENCE; SUBSET Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42360