Rishmawi, Louai, Sun, Hequan, Schneeberger, Korbinian, Huelskamp, Martin and Schrader, Andrea ORCID: 0000-0002-3879-7057
(2014).
Rapid Identification of a Natural Knockout Allele of ARMADILLO REPEAT-CONTAINING KINESIN1 That Causes Root Hair Branching by Mapping-By-Sequencing.
Plant Physiol., 166 (3).
S. 1280 - 1338.
ROCKVILLE:
AMER SOC PLANT BIOLOGISTS.
ISSN 1532-2548
Abstract
In Arabidopsis (Arabidopsis thaliana), branched root hairs are an indicator of defects in root hair tip growth. Among 62 accessions, one accession (Heiligkreuztal2 [HKT2.4]) displayed branched root hairs, suggesting that this accession carries a mutation in a gene of importance for tip growth. We determined 200- to 300-kb mapping intervals using a mapping-by-sequencing approach of F2 pools from crossings of HKT2.4 with three different accessions. The intersection of these mapping intervals was 80 kb in size featuring not more than 36 HKT2.4-specific single nucleotide polymorphisms, only two of which changed the coding potential of genes. Among them, we identified the causative single nucleotide polymorphism changing a splicing site in ARMADILLO REPEAT-CONTAINING KINESIN1. The applied strategies have the potential to complement statistical methods in high-throughput phenotyping studies using different natural accessions to identify causative genes for distinct phenotypes represented by only one or a few accessions.
Item Type: | Journal Article | ||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-424328 | ||||||||||||||||||||||||
DOI: | 10.1104/pp.114.244046 | ||||||||||||||||||||||||
Journal or Publication Title: | Plant Physiol. | ||||||||||||||||||||||||
Volume: | 166 | ||||||||||||||||||||||||
Number: | 3 | ||||||||||||||||||||||||
Page Range: | S. 1280 - 1338 | ||||||||||||||||||||||||
Date: | 2014 | ||||||||||||||||||||||||
Publisher: | AMER SOC PLANT BIOLOGISTS | ||||||||||||||||||||||||
Place of Publication: | ROCKVILLE | ||||||||||||||||||||||||
ISSN: | 1532-2548 | ||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||
Faculty: | Faculty of Mathematics and Natural Sciences | ||||||||||||||||||||||||
Divisions: | Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute | ||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||
Uncontrolled Keywords: |
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Refereed: | Yes | ||||||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/42432 |
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