Universität zu Köln

Rishmawi, Louai, Sun, Hequan, Schneeberger, Korbinian, Huelskamp, Martin and Schrader, Andrea ORCID: 0000-0002-3879-7057 (2014). Rapid Identification of a Natural Knockout Allele of ARMADILLO REPEAT-CONTAINING KINESIN1 That Causes Root Hair Branching by Mapping-By-Sequencing. Plant Physiol., 166 (3). S. 1280 - 1338. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

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Abstract

In Arabidopsis (Arabidopsis thaliana), branched root hairs are an indicator of defects in root hair tip growth. Among 62 accessions, one accession (Heiligkreuztal2 [HKT2.4]) displayed branched root hairs, suggesting that this accession carries a mutation in a gene of importance for tip growth. We determined 200- to 300-kb mapping intervals using a mapping-by-sequencing approach of F2 pools from crossings of HKT2.4 with three different accessions. The intersection of these mapping intervals was 80 kb in size featuring not more than 36 HKT2.4-specific single nucleotide polymorphisms, only two of which changed the coding potential of genes. Among them, we identified the causative single nucleotide polymorphism changing a splicing site in ARMADILLO REPEAT-CONTAINING KINESIN1. The applied strategies have the potential to complement statistical methods in high-throughput phenotyping studies using different natural accessions to identify causative genes for distinct phenotypes represented by only one or a few accessions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rishmawi, Louai UNSPECIFIED UNSPECIFIED UNSPECIFIED Sun, Hequan UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneeberger, Korbinian UNSPECIFIED UNSPECIFIED UNSPECIFIED Huelskamp, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schrader, Andrea UNSPECIFIED orcid.org/0000-0002-3879-7057 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-424328
DOI:	10.1104/pp.114.244046
Journal or Publication Title:	Plant Physiol.
Volume:	166
Number:	3
Page Range:	S. 1280 - 1338
Date:	2014
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-2548
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS-THALIANA; TIP GROWTH; MUTATION IDENTIFICATION; MESSENGER-RNA; SHORT READS; ACTIN; MORPHOGENESIS; RECOMBINATION; POPULATIONS; REVEALS Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/42432