Universität zu Köln

Mehanna, Ali, Szpotowicz, Emanuela, Schachner, Melitta and Jakovcevski, Igor ORCID: 0000-0001-7673-9600 (2014). Improved regeneration after femoral nerve injury in mice lacking functional T- and B-lymphocytes. Exp. Neurol., 261. S. 147 - 156. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2430

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Abstract

The immune system plays important functional roles in regeneration after injury to the mammalian central and peripheral nervous systems. After damage to the peripheral nerve several types of immune cells, invade the nerve within hours after the injury. To gain insights into the contribution of T- and B-lymphocytes to recovery from injury we used the mouse femoral nerve injury paradigm. RAG2-/- mice lacking mature T- and B-lymphocytes due to deletion of the recombination activating gene 2 were subjected to resection and surgical reconstruction the femoral nerve, with the wild-type mice of the same inbred genetic background serving as controls. According to single frame motion analyses, RAG2-/- mice showed better motor recovery in comparison to control mice at four and eight weeks after injury. Retrograde tracing of regrown/sprouted axons of spinal motoneurons showed increased numbers of correctly projecting motoneurons in the lumbar spinal cord of RAG2-/- mice compared with controls. Whereas there was no difference in the motoneuron soma size between genotypes. RAG2-/- mice displayed fewer cholinergic and inhibitory synaptic terminals around somata of spinal motoneurons both prior to and after injury. compared with wild-type mice. Extent of myelination of regrown axons in the motor branch of the femoral nerve measured as g-ratio was more extensive in RAG2-/- than in control mice eight weeks after injury. We conclude that activated T- and B-lymphocytes restrict motor recovery after femoral nerve injury. associated with the increased survival of motoneurons and improved remyelination. (C) 2014 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mehanna, Ali UNSPECIFIED UNSPECIFIED UNSPECIFIED Szpotowicz, Emanuela UNSPECIFIED UNSPECIFIED UNSPECIFIED Schachner, Melitta UNSPECIFIED UNSPECIFIED UNSPECIFIED Jakovcevski, Igor UNSPECIFIED orcid.org/0000-0001-7673-9600 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-424886
DOI:	10.1016/j.expneurol.2014.06.012
Journal or Publication Title:	Exp. Neurol.
Volume:	261
Page Range:	S. 147 - 156
Date:	2014
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1090-2430
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADHESION MOLECULE L1; SPINAL-CORD-INJURY; L2/HNK-1 CARBOHYDRATE; MOTOR NERVES; RECOVERY; MOUSE; REINNERVATION; EXPRESSION; RAT; PLASTICITY Multiple languages Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42488