Tomanov, Konstantin, Zeschmann, Anja, Hermkes, Rebecca, Eifler, Karolin, Ziba, Ionida, Grieco, Michele ORCID: 0000-0002-2418-5738, Novatchkova, Maria, Hofmann, Kay ORCID: 0000-0002-2289-9083, Hesse, Holger and Bachmair, Andreas ORCID: 0000-0001-7731-1841 (2014). Arabidopsis PIAL1 and 2 Promote SUMO Chain Formation as E4-Type SUMO Ligases and Are Involved in Stress Responses and Sulfur Metabolism. Plant Cell, 26 (11). S. 4547 - 4561. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-298X

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Abstract

The Arabidopsis thaliana genes PROTEIN INHIBITOR OF ACTIVATED STAT LIKE1 (PIAL1) and PIAL2 encode proteins with SP-RING domains, which occur in many ligases of the small ubiquitin-related modifier (SUMO) conjugation pathway. We show that PIAL1 and PIAL2 function as SUMO ligases capable of SUMO chain formation and require the SUMO-modified SUMO-conjugating enzyme SCE1 for optimal activity. Mutant analysis indicates a role for PIAL1 and 2 in salt stress and osmotic stress responses, whereas under standard conditions, the mutants show close to normal growth. Mutations in PIAL1 and 2 also lead to altered sulfur metabolism. We propose that, together with SUMO chain binding ubiquitin ligases, these enzymes establish a pathway for proteolytic removal of sumoylation substrates.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Tomanov, KonstantinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zeschmann, AnjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hermkes, RebeccaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eifler, KarolinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ziba, IonidaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grieco, MicheleUNSPECIFIEDorcid.org/0000-0002-2418-5738UNSPECIFIED
Novatchkova, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hofmann, KayUNSPECIFIEDorcid.org/0000-0002-2289-9083UNSPECIFIED
Hesse, HolgerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bachmair, AndreasUNSPECIFIEDorcid.org/0000-0001-7731-1841UNSPECIFIED
URN: urn:nbn:de:hbz:38-425367
DOI: 10.1105/tpc.114.131300
Journal or Publication Title: Plant Cell
Volume: 26
Number: 11
Page Range: S. 4547 - 4561
Date: 2014
Publisher: AMER SOC PLANT BIOLOGISTS
Place of Publication: ROCKVILLE
ISSN: 1532-298X
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
E3 LIGASE; GAS-CHROMATOGRAPHY; CHLOROPHYLL FLUORESCENCE; PROTEIN MODIFICATION; AMINO-ACIDS; UBIQUITIN; SUMOYLATION; SIZ1; CONJUGATION; TOLERANCEMultiple languages
Biochemistry & Molecular Biology; Plant Sciences; Cell BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/42536

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