Universität zu Köln

Gao, Jie ORCID: 0000-0002-5229-9249, Schatton, Desiree, Martinelli, Paola, Hansen, Henriette, Pla-Martin, David, Barth, Esther, Becker, Christian, Altmueller, Janine, Frommolt, Peter ORCID: 0000-0002-1966-8014, Sardiello, Marco and Rugarli, Elena I. ORCID: 0000-0002-5782-1067 (2014). CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins. J. Cell Biol., 207 (2). S. 213 - 224. NEW YORK: ROCKEFELLER UNIV PRESS. ISSN 1540-8140

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Abstract

Mitochondrial function requires coordination of two genomes for protein biogenesis, efficient quality control mechanisms, and appropriate distribution of the organelles within the cell. How these mechanisms are integrated is currently not understood. Loss of the Clu1/CluA homologue (CLUH) gene led to clustering of the mitochondrial network by an unknown mechanism. We find that CLUH is coregulated both with genes encoding mitochondrial proteins and with genes involved in ribosomal biogenesis and translation. Our functional analysis identifies CLUH as a cytosolic messenger ribonucleic acid (RNA; mRNA) binding protein. RNA immunoprecipitation experiments followed by next-generation sequencing demonstrated that CLUH specifically binds a subset of mRNAs encoding mitochondrial proteins. CLUH depletion decreased the levels of proteins translated by target transcripts and caused mitochondrial clustering. A fraction of CLUH colocalizes with tyrosinated tubulin and can be detected close to mitochondria, suggesting a role in regulating transport or translation of target transcripts close to mitochondria. Our data unravel a novel mechanism linking mitochondrial biogenesis and distribution.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gao, Jie UNSPECIFIED orcid.org/0000-0002-5229-9249 UNSPECIFIED Schatton, Desiree UNSPECIFIED UNSPECIFIED UNSPECIFIED Martinelli, Paola UNSPECIFIED UNSPECIFIED UNSPECIFIED Hansen, Henriette UNSPECIFIED UNSPECIFIED UNSPECIFIED Pla-Martin, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Barth, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Frommolt, Peter UNSPECIFIED orcid.org/0000-0002-1966-8014 UNSPECIFIED Sardiello, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Rugarli, Elena I. UNSPECIFIED orcid.org/0000-0002-5782-1067 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-425510
DOI:	10.1083/jcb.201403129
Journal or Publication Title:	J. Cell Biol.
Volume:	207
Number:	2
Page Range:	S. 213 - 224
Date:	2014
Publisher:	ROCKEFELLER UNIV PRESS
Place of Publication:	NEW YORK
ISSN:	1540-8140
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SACCHAROMYCES-CEREVISIAE; EXPRESSION PROFILES; LOCALIZATION; PARKIN; DROSOPHILA-PINK1; DIFFERENTIATION; DICTYOSTELIUM; MORPHOLOGY; MITOPHAGY; DATABASE Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/42551