Universität zu Köln

Ruthard, Johannes, Kamper, Matthias, Renno, Joerg H., Kuehn, Gertrud, Hillebrand, Ute, Hoellriegl, Stefan, Johannis, Wibke, Zaucke, Frank and Klatt, Andreas R. (2014). COMP does not directly modify the expression of genes involved in cartilage homeostasis in contrast to several other cartilage matrix proteins. Connect. Tissue Res., 55 (5-6). S. 348 - 357. LONDON: INFORMA HEALTHCARE. ISSN 1607-8438

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Abstract

Objective: We investigated whether COMP may modify cartilage metabolism and play a role as an endogenous disease aggravating factor in OA. Materials and methods: Full-length and momomeric COMP was recombinantly expressed in human embryonic kidney cells and purified it via affinity chromatography. Purified COMP was used to stimulate either primary human chondrocytes or cartilage explants. Changes in the expression profiles of inflammatory genes, differentiation markers and growth factors were examined by immunoassay and by quantitative real-time reverse-transcription polymerase chain reaction. Results: Incubation of primary human chondrocytes or cartilage explants in the presence of COMP did not induce statistically significant changes in the expression of IL-6, MMP1, MMP13, collagen I, collagen II, collagen X, TGF-beta 1 and BMP-2. Conclusions: In contrast to collagen II and matrilin-3, COMP lacks the ability to trigger a proinflammatory response in chondrocytes, although it carries an RGD motif and can bind to integrins. COMP is a well-accepted biomarker for osteoarthritis but increased COMP levels do not necessarily correlate with inflammation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ruthard, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Kamper, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Renno, Joerg H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuehn, Gertrud UNSPECIFIED UNSPECIFIED UNSPECIFIED Hillebrand, Ute UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoellriegl, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Johannis, Wibke UNSPECIFIED UNSPECIFIED UNSPECIFIED Zaucke, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Klatt, Andreas R. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-426419
DOI:	10.3109/03008207.2014.951440
Journal or Publication Title:	Connect. Tissue Res.
Volume:	55
Number:	5-6
Page Range:	S. 348 - 357
Date:	2014
Publisher:	INFORMA HEALTHCARE
Place of Publication:	LONDON
ISSN:	1607-8438
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN ARTICULAR CHONDROCYTES; MULTIPLE EPIPHYSEAL DYSPLASIA; FIBRONECTIN FRAGMENTS; SYNOVIAL-FLUID; KNEE OSTEOARTHRITIS; COLLAGEN-II; ALPHA-5-BETA-1 INTEGRIN; RHEUMATOID-ARTHRITIS; TISSUE DISTRIBUTION; CELL ATTACHMENT Multiple languages Cell Biology; Orthopedics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42641