Universität zu Köln

Molcanyi, Marek, Mehrjardi, Narges Zare, Schaefer, Ute, Haj-Yasein, Nadia Nabil, Brockmann, Michael, Penner, Marina, Riess, Peter, Reinshagen, Clemens ORCID: 0000-0002-8524-4578, Rieger, Bernhard, Hannes, Tobias ORCID: 0000-0002-8550-2282, Hescheler, Juergen and Bosche, Bert ORCID: 0000-0002-5313-3195 (2014). Impurity of stem cell graft by murine embryonic fibroblasts - implications for cell-based therapy of the central nervous system. Front. Cell. Neurosci., 8. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1662-5102

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Abstract

Stem cells have been demonstrated to possess a therapeutic potential in experimental models of various central nervous system disorders, including stroke. The types of implanted cells appear to play a crucial role. Previously, groups of the stem cell network NRW implemented a feeder-based cell line within the scope of their projects, examining the implantation of stem cells after ischemic stroke and traumatic brain injury. Retrospective evaluation indicated the presence of spindle-shaped cells in several grafts implanted in injured animals, which indicated potential contamination by co-cultured feeder cells (murine embryonic fibroblasts - MEFs). Because feeder-based cell lines have been previously exposed to a justified criticism with regard to contamination by animal glycans, we aimed to evaluate the effects of stem cell/MEF co-transplantation. MEFs accounted for 5.3 +/- 2.8% of all cells in the primary FACS-evaluated co-culture. Depending on the culture conditions and subsequent purification procedure, the MEF-fraction ranged from 0.9 to 9.9% of the cell suspensions in vitro. MEF survival and related formation of extracellular substances in vivo were observed after implantation into the uninjured rat brain. Impurity of the stem cell graft by MEFs interferes with translational strategies, which represents a threat to the potential recipient and may affect the graft microenvironment. The implications of these findings are critically discussed.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Molcanyi, Marek UNSPECIFIED UNSPECIFIED UNSPECIFIED Mehrjardi, Narges Zare UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaefer, Ute UNSPECIFIED UNSPECIFIED UNSPECIFIED Haj-Yasein, Nadia Nabil UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmann, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Penner, Marina UNSPECIFIED UNSPECIFIED UNSPECIFIED Riess, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Reinshagen, Clemens UNSPECIFIED orcid.org/0000-0002-8524-4578 UNSPECIFIED Rieger, Bernhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Hannes, Tobias UNSPECIFIED orcid.org/0000-0002-8550-2282 UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Bosche, Bert UNSPECIFIED orcid.org/0000-0002-5313-3195 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-428934
DOI:	10.3389/fncel.2014.00257
Journal or Publication Title:	Front. Cell. Neurosci.
Volume:	8
Date:	2014
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1662-5102
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRAUMATIC BRAIN-INJURY; PARKINSONS-DISEASE; DOPAMINE NEURONS; NEURAL DIFFERENTIATION; RAPID DIFFERENTIATION; EXPERIMENTAL STROKE; ISCHEMIC BRAIN; FEEDER CELLS; RAT-BRAIN; IPS CELLS Multiple languages Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42893