Universität zu Köln

Ghodsizad, Ali, Bordel, Viktor, Wiedensohler, Herrman, Elbanayosy, Ali, Koerner, Michael Martin, Berjon, Jose M. Gonzalez, Barrios, Roberto, Farag, Mina, Zeriouh, Mohamad, Loebe, Matthias, Noon, George Peter, Koegler, Gesine, Karck, Matthias and Ruhparwar, Arjang (2014). Magnetically Guided Recellularization of Decellularized Stented Porcine Pericardium-Derived Aortic Valve for TAVI. Asaio J., 60 (5). S. 582 - 587. PHILADELPHIA: LIPPINCOTT WILLIAMS & WILKINS. ISSN 1538-943X

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Abstract

Application of somatic stem cells for growth, proliferation, and differentiation in a three-dimensional pattern is an important aspect in tissue engineering. Here, we report on our bioreactor, which we applied for magnetically guided recellularization of nitinol-stented valve. Human-derived unrestricted somatic stem cells were cultured in medium in our pulsatile dynamic bioreactor for 4-6 days. Stented valves were prepared by decellularization of porcine pericardium and construction of stented tissue-engineered valves (n = 8). A magnetic field was created around the bioreactor to prevent the loss of cells. In the control group, no magnetic device was used (n = 4). Morphological characterization was assessed by immunohistochemical staining of paraffin sections and electron microscopy. The bioreactor enabled the preservation of physiologic culture conditions with aerobic cell metabolism and physiological pH values. Histological analysis showed homogeneous seeding of the pericardium with progenitor cells in the recellularized samples, whereas no cell seeding could be observed in the nonmagnetic group. Our magnetically guided multifunctional bioreactor allows for an efficient three-dimensional culturing of somatic stem cells on decellularized organ-specific matrix.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ghodsizad, Ali UNSPECIFIED UNSPECIFIED UNSPECIFIED Bordel, Viktor UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiedensohler, Herrman UNSPECIFIED UNSPECIFIED UNSPECIFIED Elbanayosy, Ali UNSPECIFIED UNSPECIFIED UNSPECIFIED Koerner, Michael Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Berjon, Jose M. Gonzalez UNSPECIFIED UNSPECIFIED UNSPECIFIED Barrios, Roberto UNSPECIFIED UNSPECIFIED UNSPECIFIED Farag, Mina UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeriouh, Mohamad UNSPECIFIED UNSPECIFIED UNSPECIFIED Loebe, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Noon, George Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Koegler, Gesine UNSPECIFIED UNSPECIFIED UNSPECIFIED Karck, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruhparwar, Arjang UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-430079
DOI:	10.1097/MAT.0000000000000110
Journal or Publication Title:	Asaio J.
Volume:	60
Number:	5
Page Range:	S. 582 - 587
Date:	2014
Publisher:	LIPPINCOTT WILLIAMS & WILKINS
Place of Publication:	PHILADELPHIA
ISSN:	1538-943X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RESPIRATORY-DISTRESS-SYNDROME; EXTRACORPOREAL CO2 REMOVAL; INDUCED LUNG INJURY; ACUTE KIDNEY INJURY; CARBON-DIOXIDE REMOVAL; SYSTEM ORGAN FAILURE; ACUTE-RENAL-FAILURE; MECHANICAL VENTILATION; PROTECTIVE VENTILATION; CONSENSUS CONFERENCE Multiple languages Engineering, Biomedical; Transplantation Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43007