Universität zu Köln

Ma, Liqing, Sotiriadou, Isaia, Cai, Qigang, Karanis, Gabriele, Wang, Geping, Wang, Guanghua, Lu, Yan, Li, Xiuping and Karanis, Panagiotis (2014). Detection of Cryptosporidium and Giardia in agricultural and water environments in the Qinghai area of China by IFT and PCR. Parasitol. Res., 113 (9). S. 3177 - 3185. NEW YORK: SPRINGER. ISSN 1432-1955

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Abstract

Qinghai Province in northwest China is strongly influenced by agricultural activities and is an important source of food and drinking water. Here, we present findings regarding the occurrence and molecular epidemiology of Cryptosporidium and Giardia species based on a large-scale investigation of areas of Qinghai Province. The diagnosis and molecular detection of Cryptosporidium oocysts and Giardia cysts was carried out using immunofluorescence microscopy (IFT), whereas nested polymerase chain reaction (PCR) in fecal smears and water samples was used for the detection and molecular characterization of the species. In total, 561 samples (260 water samples and 301 fecal samples from animals) were collected and analyzed. Of the 260 water samples, 66 samples were Cryptosporidium-positive by IFT and 71 samples were positive by nested PCR; in addition, 39 samples were Giardia-positive by IFT and 40 samples were positive by nested PCR. Of the 301 fecal samples from animals, 98 samples were Cryptosporidium-positive by IFT and 61 samples were positive by nested PCR, whereas 52 samples were Giardia-positive by IFT and 31 samples were positive by nested PCR. We showed that the water supplies and animals investigated contained Cryptosporidium and Giardia (oo)cysts. Thus, we recommend that the Chinese Government and Chinese health authorities undertake control measures to protect the food and drinking water sources in Qinghai from these pathogenic protozoa.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ma, Liqing UNSPECIFIED UNSPECIFIED UNSPECIFIED Sotiriadou, Isaia UNSPECIFIED UNSPECIFIED UNSPECIFIED Cai, Qigang UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Geping UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Guanghua UNSPECIFIED UNSPECIFIED UNSPECIFIED Lu, Yan UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Xiuping UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-430742
DOI:	10.1007/s00436-014-3979-5
Journal or Publication Title:	Parasitol. Res.
Volume:	113
Number:	9
Page Range:	S. 3177 - 3185
Date:	2014
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-1955
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTOZOAN PARASITES; NESTED PCR; OOCYSTS; SURFACE; TRANSMISSION; OUTBREAKS; SUPPLIES; CYSTS; SPP.; RAW Multiple languages Parasitology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43074