Universität zu Köln

Oberwallner, Barbara, Brodarac, Andreja, Choi, Yeong-Hoon, Saric, Tomo ORCID: 0000-0001-8344-1095, Anic, Petra, Morawietz, Lars and Stamm, Christof (2014). Preparation of cardiac extracellular matrix scaffolds by decellularization of human myocardium. J. Biomed. Mater. Res. Part A, 102 (9). S. 3263 - 3273. HOBOKEN: WILEY. ISSN 1552-4965

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Abstract

Extracellular matrix (ECM) derived by tissue decellularization has applications as a tissue engineering scaffold and for support of cellular regeneration. Myocardial ECM from animals has been produced by whole-organ perfusion or immersion processes, but methods for preparation of human myocardial ECM for therapy and research have not been compared in detail, yet. We analyzed the impact of decellularization processes on human myocardial ECM, and tested its ability to serve as a scaffold for cell seeding. Sodium dodecyl sulfate (SDS)-based decellularization, but not treatments based on Triton X-100, deoxycholate or hypo/hypertonic incubations, removed cells satisfactorily, and incubation with fetal bovine serum (FBS) eliminated residual DNA. ECM architecture was best preserved by a protocol consisting of 2 h lysis, 6 h SDS, and 3 h FBS, but age and pathology of the donor tissue are highly important for producing reproducible, high-quality scaffolds. We also studied ECM repopulation with mesenchymal stem cells (CB-MSC), cardiomyocytes derived from induced pluripotent stem cells (iPS-CM), and naive neonatal mouse cardiomyocytes. Cells attached to the matrix and proliferated and displayed higher viability than in standard culture. We conclude that human cardiac ECM sheets may be suitable scaffold for cell-matrix interaction studies and as a biomaterial for tissue regeneration and engineering. (C) 2013 Wiley Periodicals, Inc.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Oberwallner, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Brodarac, Andreja UNSPECIFIED UNSPECIFIED UNSPECIFIED Choi, Yeong-Hoon UNSPECIFIED UNSPECIFIED UNSPECIFIED Saric, Tomo UNSPECIFIED orcid.org/0000-0001-8344-1095 UNSPECIFIED Anic, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Morawietz, Lars UNSPECIFIED UNSPECIFIED UNSPECIFIED Stamm, Christof UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-431762
DOI:	10.1002/jbm.a.35000
Journal or Publication Title:	J. Biomed. Mater. Res. Part A
Volume:	102
Number:	9
Page Range:	S. 3263 - 3273
Date:	2014
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1552-4965
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MESENCHYMAL STEM-CELLS; SOURCE ANIMAL AGE; MECHANICAL FUNCTION; HEART-DISEASE; BONE-MARROW; TISSUE; PATCH; THERAPY; PLATFORM; COLLAGEN Multiple languages Engineering, Biomedical; Materials Science, Biomaterials Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43176