Universität zu Köln

Tretzel, Laura, Thomas, Andreas ORCID: 0000-0003-1199-0743, Geyer, Hans, Gmeiner, Guenter, Forsdahl, Guro, Pop, Valentin, Schaenzer, Wilhelm and Thevis, Mario (2014). Use of dried blood spots in doping control analysis of anabolic steroid esters. J. Pharm. Biomed. Anal., 96. S. 21 - 31. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1873-264X

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Abstract

Dried blood spot (DBS) sampling, a technique for whole blood sampling on a piece of filter paper, has more than 50-years tradition, particularly in the diagnostic analysis of metabolic disorders in neonatal screening. Due to the minimal invasiveness, straightforwardness, robustness against manipulation and fastness DBS sampling recommends itself as an advantageous technique in doping control analysis. The present approach highlights the development of a screening assay for the analysis of eight anabolic steroid esters (nandrolone phenylpropionate, trenbolone enanthate, testosterone acetate, testosterone cypionate, testosterone isocaproate, testosterone phenylpropionate, testosterone decanoate and testosterone undecanoate) and nandrolone in DBS. The detection of the intact esters allows an unequivocal proof of the administration of conjugates of exogenous testosterone and its derivatives. Precise, specific and linear conditions were obtained by means of liquid chromatography high resolution/high accuracy mass spectrometry. Sensitivity in the low ppb range was accomplished by the preparation of the methyloxime derivatives of the target compounds. Labeled internal standards (d(3)-nandrolone, d(3)-nandrolone caproate and d(3)-nandrolone undecanoate) were applied to compensate for the broad range in chain length of the esters. The assay presented here outlines the application of DBS for the analysis of anabolic steroid esters in doping controls for the first time providing great potential to simplify the proof of exogenous administration of testosterone. (C) 2014 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tretzel, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED orcid.org/0000-0003-1199-0743 UNSPECIFIED Geyer, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Gmeiner, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED Forsdahl, Guro UNSPECIFIED UNSPECIFIED UNSPECIFIED Pop, Valentin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaenzer, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-432173
DOI:	10.1016/j.jpba.2014.03.013
Journal or Publication Title:	J. Pharm. Biomed. Anal.
Volume:	96
Page Range:	S. 21 - 31
Date:	2014
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1873-264X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COLLISION-INDUCED DISSOCIATION; ELECTROSPRAY MASS-SPECTROMETRY; ORAL TESTOSTERONE UNDECANOATE; FILTER-PAPER; HUMAN-PLASMA; LC-MS/MS; DBS; QUANTIFICATION; VALIDATION; PROPIONATE Multiple languages Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43217