Universität zu Köln

Heras-Bautista, Carlos O., Katsen-Globa, Alisa, Schloerer, Nils E., Dieluweit, Sabine ORCID: 0000-0002-8640-9063, Abd El Aziz, Osama M., Peinkofer, Gabriel, Attia, Wael A., Khalil, Markus, Brockmeier, Konrad, Hescheler, Juergen and Pfannkuche, Kurt (2014). The influence of physiological matrix conditions on permanent culture of induced pluripotent stem cell-derived cardiomyocytes. Biomaterials, 35 (26). S. 7374 - 7386. OXFORD: ELSEVIER SCI LTD. ISSN 1878-5905

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Abstract

Cardiomyocytes (CMs) from induced pluripotent stem (iPS) cells mark an important achievement in the development of in vitro pharmacological, toxicological and developmental assays and in the establishment of protocols for cardiac cell replacement therapy. Using CMs generated from murine embryonic stem cells and iPS cells we found increased cell-matrix interaction and more matured embryoid body (EB) structures in iPS cell-derived EBs. However, neither suspension-culture in form of purified cardiac clusters nor adherence-culture on traditional cell culture plastic allowed for extended culture of CMs. CMs grown for five weeks on polystyrene exhibit signs of massive mechanical stress as indicated by alpha-smooth muscle actin expression and loss of sarcomere integrity. Hydrogels from polyacrylamide allow adapting of the matrix stiffness to that of cardiac tissue. We were able to eliminate the bottleneck of low cell adhesion using 2,5-Dioxopyrrolidin-1-yl-6-acrylamidohexanoate as a crosslinker to immobilize matrix proteins on the gels surface. Finally we present an easy method to generate polyacrylamide gels with a physiological Young's modulus of 55 kPa and defined surface ligand, facilitating the culture of murine and human iPS-CMs, removing excess mechanical stresses and reducing the risk of tissue culture artifacts exerted by stiff substrates. (C) 2014 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Heras-Bautista, Carlos O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Katsen-Globa, Alisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Schloerer, Nils E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dieluweit, Sabine UNSPECIFIED orcid.org/0000-0002-8640-9063 UNSPECIFIED Abd El Aziz, Osama M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Peinkofer, Gabriel UNSPECIFIED UNSPECIFIED UNSPECIFIED Attia, Wael A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Khalil, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmeier, Konrad UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfannkuche, Kurt UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-433151
DOI:	10.1016/j.biomaterials.2014.05.027
Journal or Publication Title:	Biomaterials
Volume:	35
Number:	26
Page Range:	S. 7374 - 7386
Date:	2014
Publisher:	ELSEVIER SCI LTD
Place of Publication:	OXFORD
ISSN:	1878-5905
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ATOMIC-FORCE MICROSCOPE; MECHANICAL-PROPERTIES; CARDIAC MYOCYTES; FOCAL ADHESIONS; HEART-CELLS; FIBROBLASTS; SUBSTRATE; DIFFERENTIATE; ENGRAFTMENT; LOCOMOTION Multiple languages Engineering, Biomedical; Materials Science, Biomaterials Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43315