Universität zu Köln

Karani, M., Sotiriadou, I., Plutzer, J. and Karanis, P. (2014). Bench-scale experiments for the development of a unified loop-mediated isothermal amplification (LAMP) assay for the in vitro diagnosis of Leishmania species' promastigotes. Epidemiol. Infect., 142 (8). S. 1671 - 1678. NEW YORK: CAMBRIDGE UNIV PRESS. ISSN 1469-4409

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Abstract

We developed, in bench-scale experiments, a unified loop-mediated isothermal amplification (LAMP) assay for the detection of cutaneous, mucocutaneous and visceral leishmaniasis using DNA of cultivated promastigotes. Two primer sets for the LAMP assay were designed based on the 18S rRNA gene, and their sensitivity and specificity were tested and compared. Both of them were specific for Leishmania as the DNA of all ten Leishmania species tested was amplified, whereas the DNA of other parasites, including that of Trypanosoma, was not. The detection limit for primer set 1 ranged between 30 pg and 3.6 fg, depending on which Leishmania species tested. Primer set 2 showed high sensitivity, but was less sensitive than primer set 1. Our findings lead to the conclusion that the LAMP assay with primer set 1 is a promising and effective assay for the successful detection of a wide range of Leishmania infections using only a unified multiplex LAMP test.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Karani, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sotiriadou, I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Plutzer, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, P. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-433549
DOI:	10.1017/S0950268813002677
Journal or Publication Title:	Epidemiol. Infect.
Volume:	142
Number:	8
Page Range:	S. 1671 - 1678
Date:	2014
Publisher:	CAMBRIDGE UNIV PRESS
Place of Publication:	NEW YORK
ISSN:	1469-4409
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLYMERASE-CHAIN-REACTION; SENSITIVE DETECTION; IMMUNE-RESPONSE; WATER SAMPLES; PARASITES; CRYPTOSPORIDIUM; INFECTION; DNA Multiple languages Public, Environmental & Occupational Health; Infectious Diseases Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43354