Kelly, Bernard T., Graham, Stephen C., Liska, Nicole, Dannhauser, Philip N., Hoening, Stefan, Ungewickell, Ernst J. and Owen, David J. (2014). AP2 controls clathrin polymerization with a membrane-activated switch. Science, 345 (6195). S. 459 - 464. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 1095-9203
Full text not available from this repository.Abstract
Clathrin-mediated endocytosis (CME) is vital for the internalization of most cell-surface proteins. In CME, plasma membrane-binding clathrin adaptors recruit and polymerize clathrin to form clathrin-coated pits into which cargo is sorted. Assembly polypeptide 2 (AP2) is the most abundant adaptor and is pivotal to CME. Here, we determined a structure of AP2 that includes the clathrin-binding beta 2 hinge and developed an AP2-dependent budding assay. Our findings suggest that an autoinhibitory mechanism prevents clathrin recruitment by cytosolic AP2. A large-scale conformational change driven by the plasma membrane phosphoinositide phosphatidylinositol 4,5-bisphosphate and cargo relieves this autoinhibition, triggering clathrin recruitment and hence clathrin-coated bud formation. This molecular switching mechanism can couple AP2's membrane recruitment to its key functions of cargo and clathrin binding.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-433709 | ||||||||||||||||||||||||||||||||
DOI: | 10.1126/science.1254836 | ||||||||||||||||||||||||||||||||
Journal or Publication Title: | Science | ||||||||||||||||||||||||||||||||
Volume: | 345 | ||||||||||||||||||||||||||||||||
Number: | 6195 | ||||||||||||||||||||||||||||||||
Page Range: | S. 459 - 464 | ||||||||||||||||||||||||||||||||
Date: | 2014 | ||||||||||||||||||||||||||||||||
Publisher: | AMER ASSOC ADVANCEMENT SCIENCE | ||||||||||||||||||||||||||||||||
Place of Publication: | WASHINGTON | ||||||||||||||||||||||||||||||||
ISSN: | 1095-9203 | ||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/43370 |
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