Shimabukuro-Vornhagen, Alexander ORCID: 0000-0002-2351-7294, Schloesser, Hans A., Gryschok, Luise, Malcher, Joke, Wennhold, Kerstin ORCID: 0000-0001-5539-7226, Garcia-Marquez, Maria, Herbold, Till, Neuhaus, Laura S., Becker, Hans J., Fiedler, Anne, Scherwitz, Pascal, Koslowsky, Thomas, Hake, Roland, Stippel, Dirk L., Hoelscher, Arnulf H., Eidt, Sebastian, Hallek, Michael, Theurich, Sebastian ORCID: 0000-0001-5706-8258 and von Bergwelt-Baildon, Michael S. (2014). Characterization of tumor-associated B-cell subsets in patients with colorectal cancer. Oncotarget, 5 (13). S. 4651 - 4665. ORCHARD PARK: IMPACT JOURNALS LLC. ISSN 1949-2553

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Abstract

Purpose: A precise understanding of the mechanisms by which human immune cell subsets affect tumor biology will be critical for successful treatment of cancer using immunotherapeutic approaches. Recent evidence suggests that B cells can both promote and inhibit the development and progression of tumors. The aim of this study was to characterize the composition of the B-cell infiltrates in colorectal cancers (CRC) in order to gain further insight into the role of B cells in CRC. Experimental Design: In this study we characterized B-cell subsets in primary tumors (n=38), metastases (n=6) and blood (n=46) of 51 patients with a diagnosis of CRC and blood of 10 healthy controls. B-cell subsets were analyzed by flow cytometry or immunohistochemistry. Results: Peripheral blood of CRC patients contained a higher percentage of memory B cells than that of age-matched healthy controls. Furthermore, the percentage of B cells within tumors was higher than that in the peripheral blood of CRC patients while metastases were typically devoid of tumor-infiltrating B cells. Tumor-associated B cells were enriched for activated and terminally differentiated B cells. Relevant proportions of regulatory B cells could only be detected in advanced cancer and metastases. Conclusion: B cells constitute a significant proportion of the immune infiltrate in CRC. The B-cell infiltrate of primary CRC is characterized by an accumulation of terminally differentiated memory B cells or plasma cells suggestive of a specific immune response against the tumor. However advanced tumors and metastases are also infiltrated by a considerable number of regulatory B cells.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Shimabukuro-Vornhagen, AlexanderUNSPECIFIEDorcid.org/0000-0002-2351-7294UNSPECIFIED
Schloesser, Hans A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gryschok, LuiseUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Malcher, JokeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wennhold, KerstinUNSPECIFIEDorcid.org/0000-0001-5539-7226UNSPECIFIED
Garcia-Marquez, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Herbold, TillUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Neuhaus, Laura S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Becker, Hans J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fiedler, AnneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Scherwitz, PascalUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koslowsky, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hake, RolandUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stippel, Dirk L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoelscher, Arnulf H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eidt, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hallek, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Theurich, SebastianUNSPECIFIEDorcid.org/0000-0001-5706-8258UNSPECIFIED
von Bergwelt-Baildon, Michael S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-433833
DOI: 10.18632/oncotarget.1701
Journal or Publication Title: Oncotarget
Volume: 5
Number: 13
Page Range: S. 4651 - 4665
Date: 2014
Publisher: IMPACT JOURNALS LLC
Place of Publication: ORCHARD PARK
ISSN: 1949-2553
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
HUMAN SOLID TUMORS; INFILTRATING LYMPHOCYTES; BREAST-CANCER; IMMUNE CELLS; FAVORABLE PROGNOSIS; ANTITUMOR IMMUNITY; REGULATORY CELLS; T-CELLS; MELANOMA; ANTIGENMultiple languages
Oncology; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/43383

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