Universität zu Köln

Berlth, Felix ORCID: 0000-0002-3780-0728, Moenig, Stefan P., Schloesser, Hans A., Maus, Martin, Baltin, Christoph T. H., Urbanski, Alexander, Drebber, Uta, Bollschweiler, Elfriede, Hoelscher, Arnulf H. and Alakus, Hakan (2014). Validation of 2-mm Tissue Microarray Technology in Gastric Cancer. Agreement of 2-mm TMAs and Full Sections for Glut-1 and Hif-1 Alpha. Anticancer Res., 34 (7). S. 3313 - 3321. ATHENS: INT INST ANTICANCER RESEARCH. ISSN 1791-7530

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Abstract

Background/Aim: Tissue Microarray (TMA) is a widely used method to perform high-throughput immunohistochemical analyses on different tissues by arraying small sample cores from paraffin-fixed tissues into a single paraffin block. TMA-technology has been validated on numerous cancer tissues and also for gastric cancer studies, although it has not been validated for this tumor tissue so far. The objective of this study was to assess, whether the 2-mm TMA-technology is able to provide representative samples of gastric cancer tissue. Materials and Methods: TMA paraffin blocks were constructed by means of 220 formalin-fixed and paraffin-embedded gastric cancer samples with a sample diameter of 2 mm. The agreement of immunohistochemical stainings of Glut-1 and Hif-1 alpha in TMA sections and the original full sections was calculated using kappa statistics and direct adjustment. Results: The congruence was substantial for Glut-1 (kappa 0.64) and Hif-1 alpha (kappa 0.70), but with an agreement of only 71% and 52% within the marker-positive cases of the full-section slides. Conclusion: Due to tumor heterogeneity primarily, the TMA technology with a 2-mm sample core shows relevant limitations in gastric cancer tissue. Although being helpful for tissue screening purposes, the 2-mm TMA technology cannot be recommended as a method equal to full-section investigations in gastric cancer.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Berlth, Felix UNSPECIFIED orcid.org/0000-0002-3780-0728 UNSPECIFIED Moenig, Stefan P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schloesser, Hans A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maus, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Baltin, Christoph T. H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Urbanski, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Drebber, Uta UNSPECIFIED UNSPECIFIED UNSPECIFIED Bollschweiler, Elfriede UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoelscher, Arnulf H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Alakus, Hakan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-434618
Journal or Publication Title:	Anticancer Res.
Volume:	34
Number:	7
Page Range:	S. 3313 - 3321
Date:	2014
Publisher:	INT INST ANTICANCER RESEARCH
Place of Publication:	ATHENS
ISSN:	1791-7530
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SQUAMOUS-CELL CARCINOMA; BREAST-CARCINOMA; PROTEIN EXPRESSION; IN-SITU; IMMUNOHISTOCHEMISTRY; ADENOCARCINOMA; HIF-1-ALPHA; SPECIMENS; TUMORS; ARRAY Multiple languages Oncology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43461