Universität zu Köln

Mainz, Daniela, Quadt, Ilja, Stranzenbach, Andrea K., Voss, Daniel, Guarino, Linda A. and Knebel-Moersdorf, Dagmar (2014). Expression and nuclear localization of the TATA-box-binding protein during baculovirus infection. J. Gen. Virol., 95. S. 1396 - 1408. READING: SOC GENERAL MICROBIOLOGY. ISSN 1465-2099

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Abstract

The TATA-box-binding protein (TBP) plays a key role in initiating eukaryotic transcription and is used by many viruses for viral transcription. We previously reported increased TBP levels during infection with the baculovirus Autographa californica multicapsid nuclear polyhedrovirus (AcMNPV). The TBP antiserum used in that study, however, cross-reacted with a baculoviral protein. Here, we reported that increased amounts of nuclear TBP were detected upon infection of Spodoptera frugiperda and TN-368 cells with a TBP-specific antiserum. TBP levels increased until 72 h post-infection (p.i.), whilst tbp transcripts decreased by 16 h p.i., which suggested a virus-induced influence on the TBP protein levels. To address a potential modification of the TBP degradation pathway during infection, we investigated the possible role of viral ubiquitin. Infection studies with AcMNPV recombinants carrying a mutated viral ubiquitin gene revealed that the TBP increase during infection was not altered. In addition, pulse-chase experiments indicated a high TBP half-life of similar to 60 h in uninfected cells, suggesting that a virus-induced increase of TBP stability was unlikely. This increase in TBP correlated with a redistribution to nuclear domains resembling sites of viral DNA synthesis. Furthermore, we observed colocalization of TBP with host RNA polymerase (RNAP) II, but only until 8 h p.i., whilst TBP, but not RNAPII, was present in the enlarged replication domains late during infection. Thus, we suggested that AcMNPV adapted a mechanism to accumulate the highly stable cellular TBP at sites of viral DNA replication and transcription.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mainz, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Quadt, Ilja UNSPECIFIED UNSPECIFIED UNSPECIFIED Stranzenbach, Andrea K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Voss, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Guarino, Linda A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Knebel-Moersdorf, Dagmar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-436663
DOI:	10.1099/vir.0.059949-0
Journal or Publication Title:	J. Gen. Virol.
Volume:	95
Page Range:	S. 1396 - 1408
Date:	2014
Publisher:	SOC GENERAL MICROBIOLOGY
Place of Publication:	READING
ISSN:	1465-2099
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SPODOPTERA-FRUGIPERDA CELLS; DNA REPLICATION SITES; AUTOGRAPHA-CALIFORNICA; RNA-POLYMERASE; POLYHEDROSIS-VIRUS; VIRAL-DNA; TRANSCRIPTION FACTOR; GENE-TRANSCRIPTION; TRICHOPLUSIA-NI; INSECT CELLS Multiple languages Biotechnology & Applied Microbiology; Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43666