Universität zu Köln

Marcuzzi, Gian Paolo, Awerkiew, Sabine, Hufbauer, Martin, Schaedlich, Lysann, Gissmann, Lutz, Eming, Sabine and Pfister, Herbert (2014). Tumor prevention in HPV8 transgenic mice by HPV8-E6 DNA vaccination. Med. Microbiol. Immunol., 203 (3). S. 155 - 164. NEW YORK: SPRINGER. ISSN 1432-1831

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Abstract

The genus beta human papillomavirus 8 (HPV8) is involved in the development of cutaneous squamous cell carcinomas (SCCs) in individuals with epidermodysplasia verruciformis. Immunosuppressed transplant recipients are prone to harbor particularly high betapapillomavirus DNA loads, which may contribute to their highly increased risk of SCC. Tumor induction in HPV8 transgenic mice correlates with increased expression of viral oncogenes E6 and E2. In an attempt to prevent skin tumor development, we evaluated an HPV8-E6-DNA vaccine, which was able to stimulate a detectable HPV8-E6-specific cell-mediated immune response in 8/15 immunized mice. When skin of HPV8 transgenic mice was grafted onto non-transgenic littermates, the grafted HPV8 transgenic tissue was not rejected and papillomas started to grow within 14 days all over the transplant of 9/9 non-vaccinated and 7/15 not successfully vaccinated mice. In contrast, no papillomas developed in 6/8 successfully vaccinated mice. In the other two of these eight mice, a large ulcerative lesion developed within the initial papilloma growth or papilloma development was highly delayed. As the vaccine completely or partially prevented papilloma development without rejecting the transplanted HPV8 positive skin, the immune system appears to attack only keratinocytes with increased levels of E6 protein, which would give rise to papillomas.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Marcuzzi, Gian Paolo UNSPECIFIED UNSPECIFIED UNSPECIFIED Awerkiew, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Hufbauer, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaedlich, Lysann UNSPECIFIED UNSPECIFIED UNSPECIFIED Gissmann, Lutz UNSPECIFIED UNSPECIFIED UNSPECIFIED Eming, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfister, Herbert UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-437207
DOI:	10.1007/s00430-014-0327-4
Journal or Publication Title:	Med. Microbiol. Immunol.
Volume:	203
Number:	3
Page Range:	S. 155 - 164
Date:	2014
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-1831
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN-PAPILLOMAVIRUS; BETAPAPILLOMAVIRUS INFECTION; SKIN TUMORS; CELL Multiple languages Immunology; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43720