Universität zu Köln

Mille, Bea G., Peigneur, Steve ORCID: 0000-0003-0504-5702, Diego-Garcia, Elia ORCID: 0000-0003-2408-4248, Predel, Reinhard and Tytgat, Jan ORCID: 0000-0003-1778-6022 (2014). Partial transcriptomic profiling of toxins from the venom gland of the scorpion Parabuthus stridulus. Toxicon, 83. S. 75 - 84. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 0041-0101

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Abstract

Since it is an apocrine secretion, scorpion venom is a complex mixture that contains a variety of low-molecular-weight basic proteins (neurotoxins), mucus, salts, as well as a large number of other constituents. Diversity of scorpion venom peptides exists also at the transcript level. Two kinds of venom peptides are typically considered: the neurotoxins and the antimicrobial peptides. We constructed a cDNA library and carried an EST (Expressed Sequence Tag) approach to overview the different peptides in the transcriptome of the telson from Parabuthus stridulus. P. stridulus are psammophilous and highly venomous scorpions endemic to Namibia (Prendini 2004) with medical relevance because of important human envenomation occurrence. We obtained 111 ESTs, 20% of them corresponding to cellular process transcripts, 7% to hypothetical proteins and 17% were sequences without good matches, but the majority of ESTs, 56%, corresponds to transcripts encoding for different venom components, including voltage-gated sodium, potassium and calcium channel toxins, antimicrobial peptides and other venom and cell proteins. To the best of our knowledge this report contains the first transcriptome analysis of genes transcribed by the venomous gland of the scorpion species P. stridulus, belonging to the family of medically important Buthidae scorpions. One hundred and eleven ESTs were analyzed, showing an important number of genes that encode for 'products similar to known scorpion venom components. In total, 17 unique and novel sequences were indentified. The identification and characterization of these compounds will be a good source of novel pharmacological tools for studying ion channels and the understanding of the physiological effects of toxins in P. stridulus envenomations at a molecular level. (C) 2014 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mille, Bea G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Peigneur, Steve UNSPECIFIED orcid.org/0000-0003-0504-5702 UNSPECIFIED Diego-Garcia, Elia UNSPECIFIED orcid.org/0000-0003-2408-4248 UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Tytgat, Jan UNSPECIFIED orcid.org/0000-0003-1778-6022 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-437271
DOI:	10.1016/j.toxicon.2014.03.001
Journal or Publication Title:	Toxicon
Volume:	83
Page Range:	S. 75 - 84
Date:	2014
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	0041-0101
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LIPOLYSIS ACTIVATING PEPTIDE; POTASSIUM CHANNEL BLOCKER; MOLECULAR CHARACTERIZATION; ANTIMICROBIAL PEPTIDES; DISULFIDE BRIDGE; SEQUENCE; PURIFICATION; INHIBITION; SUBFAMILY; PROTEIN Multiple languages Pharmacology & Pharmacy; Toxicology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43727