Universität zu Köln

Irving, Aaron T., Mimuro, Hitomi, Kufer, Thomas A., Lo, Camden, Wheeler, Richard, Turner, Lorinda J., Thomas, Belinda J., Malosse, Christian, Gantier, Michael P., Casillas, Linda N., Votta, Bartholomew J., Bertin, John, Boneca, Ivo G., Sasakawa, Chihiro, Philpott, Dana J., Ferrero, Richard L. and Kaparakis-Liaskos, Maria ORCID: 0000-0002-8964-4456 (2014). The Immune Receptor NOD1 and Kinase RIP2 Interact with Bacterial Peptidoglycan on Early Endosomes to Promote Autophagy and Inflammatory Signaling. Cell Host Microbe, 15 (5). S. 623 - 636. CAMBRIDGE: CELL PRESS. ISSN 1934-6069

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Abstract

The intracellular innate immune receptor NOD1 detects Gram-negative bacterial peptidoglycan (PG) to induce autophagy and inflammatory responses in host cells. To date, the intracellular compartment in which PG is detected by NOD1 and whether NOD1 directly interacts with PG are two questions that remain to be resolved. To address this, we used outer membrane vesicles (OMVs) from pathogenic bacteria as a physiological mechanism to deliver PG into the host cell cytosol. We report that OMVs induced autophagosome formation and inflammatory IL-8 responses in epithelial cells in a NOD1-and RIP2-dependent manner. PG contained within OMVs colocalized with both NOD1 and RIP2 in EEA1-positive early endosomes. Further, we provide evidence for direct interactions between NOD1 and PG. Collectively, these findings demonstrate that NOD1 detects PG within early endosomes, thereby promoting RIP2-dependent autophagy and inflammatory signaling in response to bacterial infection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Irving, Aaron T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mimuro, Hitomi UNSPECIFIED UNSPECIFIED UNSPECIFIED Kufer, Thomas A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lo, Camden UNSPECIFIED UNSPECIFIED UNSPECIFIED Wheeler, Richard UNSPECIFIED UNSPECIFIED UNSPECIFIED Turner, Lorinda J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Belinda J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Malosse, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Gantier, Michael P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Casillas, Linda N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Votta, Bartholomew J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bertin, John UNSPECIFIED UNSPECIFIED UNSPECIFIED Boneca, Ivo G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sasakawa, Chihiro UNSPECIFIED UNSPECIFIED UNSPECIFIED Philpott, Dana J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ferrero, Richard L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaparakis-Liaskos, Maria UNSPECIFIED orcid.org/0000-0002-8964-4456 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-438332
DOI:	10.1016/j.chom.2014.04.001
Journal or Publication Title:	Cell Host Microbe
Volume:	15
Number:	5
Page Range:	S. 623 - 636
Date:	2014
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1934-6069
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NF-KAPPA-B; OUTER-MEMBRANE VESICLES; HELICOBACTER-PYLORI; ACTIVATION; RECOGNITION; MOLECULE; CELL Multiple languages Microbiology; Parasitology; Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43833