Universität zu Köln

Mascher, Martin ORCID: 0000-0001-6373-6013, Gerlach, Nina, Gahrtz, Manfred, Bucher, Marcel ORCID: 0000-0003-1680-9413, Scholz, Uwe ORCID: 0000-0001-6113-3518 and Dresselhaus, Thomas (2014). Sequence and Ionomic Analysis of Divergent Strains of Maize Inbred Line B73 with an Altered Growth Phenotype. PLoS One, 9 (5). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Maize (Zea mays) is the most widely grown crop species in the world and a classical model organism for plant research. The completion of a high-quality reference genome sequence and the advent of high-throughput sequencing have greatly empowered re-sequencing studies in maize. In this study, plants of maize inbred line B73 descended from two different sets of seed material grown for several generations either in the field or in the greenhouse were found to show a different growth phenotype and ionome under phosphate starvation conditions and moreover a different responsiveness towards mycorrhizal fungi of the species Glomus intraradices (syn: Rhizophagus irregularis). Whole genome re-sequencing of individuals from both sets and comparison to the B73 reference sequence revealed three cryptic introgressions on chromosomes 1, 5 and 10 in the line grown in the greenhouse summing up to a total of 5,257 single-nucleotide polymorphisms (SNPs). Transcriptome sequencing of three individuals from each set lent further support to the location of the introgression intervals and confirmed them to be fixed in all sequenced individuals. Moreover, we identified.120 genes differentially expressed between the two B73 lines. We thus have found a nearly-isogenic line (NIL) of maize inbred line B73 that is characterized by an altered growth phenotype under phosphate starvation conditions and an improved responsiveness towards symbiosis with mycorrhizal fungi. Through next-generation sequencing of the genomes and transcriptomes we were able to delineate exact introgression intervals. Putative de novo mutations appeared approximately uniformly distributed along the ten maize chromosomes mainly representing G: C ->. A: T transitions. The plant material described in this study will be a valuable tool both for functional studies of genes differentially expressed in both B73 lines and for research on growth behavior especially in response to symbiosis between maize and mycorrhizal fungi.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mascher, Martin UNSPECIFIED orcid.org/0000-0001-6373-6013 UNSPECIFIED Gerlach, Nina UNSPECIFIED UNSPECIFIED UNSPECIFIED Gahrtz, Manfred UNSPECIFIED UNSPECIFIED UNSPECIFIED Bucher, Marcel UNSPECIFIED orcid.org/0000-0003-1680-9413 UNSPECIFIED Scholz, Uwe UNSPECIFIED orcid.org/0000-0001-6113-3518 UNSPECIFIED Dresselhaus, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-438450
DOI:	10.1371/journal.pone.0096782
Journal or Publication Title:	PLoS One
Volume:	9
Number:	5
Date:	2014
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SPONTANEOUS MUTATIONS; GENOME; ASSOCIATION; RESOURCE; MYCORRHIZA; DIVERSITY; RESPONSES; SYMBIOSIS; SPECTRUM; DATABASE Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/43845