Universität zu Köln

Kohli, Priyanka ORCID: 0000-0002-0651-4008, Bartram, Malte P., Habbig, Sandra, Pahmeyer, Caroline, Lamkemeyer, Tobias, Benzing, Thomas, Schermer, Bernhard ORCID: 0000-0002-5194-9000 and Rinschen, Markus M. (2014). Label-free quantitative proteomic analysis of the YAP/TAZ interactome. Am. J. Physiol.-Cell Physiol., 306 (9). S. C805 - 14. BETHESDA: AMER PHYSIOLOGICAL SOC. ISSN 1522-1563

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Abstract

The function of an individual protein is typically defined by protein-protein interactions orchestrating the formation of large complexes critical for a wide variety of biological processes. Over the last decade the analysis of purified protein complexes by mass spectrometry became a key technique to identify protein-protein interactions. We present a fast and straightforward approach for analyses of interacting proteins combining a Flp-in single-copy cellular integration system and single-step affinity purification with single-shot mass spectrometry analysis. We applied this protocol to the analysis of the YAP and TAZ interactome. YAP and TAZ are the downstream effectors of the mammalian Hippo tumor suppressor pathway. Our study provides comprehensive interactomes for both YAP and TAZ and does not only confirm the majority of previously described interactors but, strikingly, revealed uncharacterized interaction partners that affect YAP/TAZ TEAD-dependent transcription. Among these newly identified candidates are Rassf8, thymopoetin, and the transcription factors CCAAT/enhancer-binding protein (C/EBP)beta/delta and core-binding factor subunit beta (Cbfb). In addition, our data allowed insights into complex stoichiometry and uncovered discrepancies between the YAP and TAZ interactomes. Taken together, the stringent approach presented here could help to significantly sharpen the understanding of protein-protein networks.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kohli, Priyanka UNSPECIFIED orcid.org/0000-0002-0651-4008 UNSPECIFIED Bartram, Malte P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Habbig, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Pahmeyer, Caroline UNSPECIFIED UNSPECIFIED UNSPECIFIED Lamkemeyer, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Benzing, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Schermer, Bernhard UNSPECIFIED orcid.org/0000-0002-5194-9000 UNSPECIFIED Rinschen, Markus M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-438779
DOI:	10.1152/ajpcell.00339.2013
Journal or Publication Title:	Am. J. Physiol.-Cell Physiol.
Volume:	306
Number:	9
Page Range:	S. C805 - 14
Date:	2014
Publisher:	AMER PHYSIOLOGICAL SOC
Place of Publication:	BETHESDA
ISSN:	1522-1563
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language YES-ASSOCIATED PROTEIN; HIPPO-YAP PATHWAY; TUMOR-SUPPRESSOR; TRANSCRIPTION FACTORS; WW DOMAIN; NUCLEAR-LOCALIZATION; CONTACT INHIBITION; DEPENDENT CONTROL; BETA INTERACTS; ALPHA-CATENIN Multiple languages Cell Biology; Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43877