Universität zu Köln

Gaafar, Taghrid M., Abdel Rahman, Hala A., Attia, Wael ORCID: 0000-0001-5822-6481, Hamza, Hala S., Brockmeier, Konrad and El Hawary, Rabab E. (2014). Comparative characteristics of endothelial-like cells derived from human adipose mesenchymal stem cells and umbilical cord blood-derived endothelial cells. Clin. Exper. Med., 14 (2). S. 177 - 185. MILAN: SPRINGER-VERLAG ITALIA SRL. ISSN 1591-9528

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Abstract

Adult peripheral blood contains a limited number of endothelial progenitor cells that can be isolated for treatment of ischemic diseases. The adipose tissue became an interesting source of stem cells for regenerative medicine. This study aimed to investigate the phenotype of cells obtained by culturing adipose-derived mesenchymal stem cells (ad-MSCs) in the presence of endothelial growth supplements compared to endothelial cells obtained from umbilical cord blood (UCB). Passage 3 ad-MSCs and mononuclear layer from UCB were cultured in presence of endothelial growth media for 3 weeks followed by their characterization by flow cytometry and polymerase chain reaction. After culture in endothelial inductive media, ad-MSCs expressed endothelial genes and some endothelial marker proteins as CD31 and CD34, respectively. Adipose tissue could be a reliable source for easy obtaining, expanding and differentiating MSCs into endothelial-like cells for autologous cell-based therapy.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gaafar, Taghrid M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Abdel Rahman, Hala A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Attia, Wael UNSPECIFIED orcid.org/0000-0001-5822-6481 UNSPECIFIED Hamza, Hala S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmeier, Konrad UNSPECIFIED UNSPECIFIED UNSPECIFIED El Hawary, Rabab E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-439315
DOI:	10.1007/s10238-013-0238-5
Journal or Publication Title:	Clin. Exper. Med.
Volume:	14
Number:	2
Page Range:	S. 177 - 185
Date:	2014
Publisher:	SPRINGER-VERLAG ITALIA SRL
Place of Publication:	MILAN
ISSN:	1591-9528
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROGENITOR CELLS; BONE-MARROW; TISSUE; DIFFERENTIATION Multiple languages Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43931