Hartwig, Sonja, Raschke, Silja, Knebel, Birgit, Scheler, Mika, Irmler, Martin ORCID: 0000-0003-3169-479X, Passlack, Waltraud, Muller, Stefan, Hanisch, Franz-Georg, Franz, Thomas, Li, Xinping, Dicken, Hans-Dieter, Eckardt, Kristin, Beckers, Johannes ORCID: 0000-0001-7874-3822, de Angelis, Martin Hrabe ORCID: 0000-0002-7898-2353, Weigert, Cora, Haering, Hans-Ulrich, Al-Hasani, Hadi, Ouwens, D. Margriet, Eckel, Juergen ORCID: 0000-0003-3645-5288, Kotzka, Jorg ORCID: 0000-0003-1173-9372 and Lehr, Stefan (2014). Secretome profiling Of primary human skeletal muscle cells. BBA-Proteins Proteomics, 1844 (5). S. 1011 - 1018. AMSTERDAM: ELSEVIER. ISSN 1878-1454

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Abstract

The skeletal muscle is a metabolically active tissue that secretes various proteins. These so-called myokines have been proposed to affect muscle physiology and to exert systemic effects on other tissues and organs. Yet, changes in the secretory profile may participate in the pathophysiology of metabolic diseases. The present study, aimed at characterizing the secretome of differentiated primary human skeletal muscle cells (hSkMC) derived from healthy, adult donors combining three different mass spectrometry based non-targeted approaches as well as one antibody based method. This led to the identification of 548 non-redundant proteins in conditioned media from hSkmc. For 501 proteins, significant mRNA expression could be demonstrated. Applying stringent consecutive filtering using SignalP, SecretomeP and ER_retention signal databases, 305 proteins were assigned as potential myokines of which 12 proteins containing a secretory signal peptide were not previously described. This comprehensive profiling study of the human skeletal muscle secretome expands our knowledge of the composition of the human myokinome and may contribute to our understanding of the role of myokines in multiple biological processes. This article is part of a Special Issue entitled: Biomarkers: A Proteomic Challenge. (C) 2013 Elsevier B.V. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Hartwig, SonjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Raschke, SiljaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Knebel, BirgitUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Scheler, MikaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Irmler, MartinUNSPECIFIEDorcid.org/0000-0003-3169-479XUNSPECIFIED
Passlack, WaltraudUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Muller, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hanisch, Franz-GeorgUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Franz, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Li, XinpingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dicken, Hans-DieterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eckardt, KristinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Beckers, JohannesUNSPECIFIEDorcid.org/0000-0001-7874-3822UNSPECIFIED
de Angelis, Martin HrabeUNSPECIFIEDorcid.org/0000-0002-7898-2353UNSPECIFIED
Weigert, CoraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Haering, Hans-UlrichUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Al-Hasani, HadiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ouwens, D. MargrietUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eckel, JuergenUNSPECIFIEDorcid.org/0000-0003-3645-5288UNSPECIFIED
Kotzka, JorgUNSPECIFIEDorcid.org/0000-0003-1173-9372UNSPECIFIED
Lehr, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-439836
DOI: 10.1016/j.bbapap.2013.08.004
Journal or Publication Title: BBA-Proteins Proteomics
Volume: 1844
Number: 5
Page Range: S. 1011 - 1018
Date: 2014
Publisher: ELSEVIER
Place of Publication: AMSTERDAM
ISSN: 1878-1454
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
EXPRESSION; DIFFERENTIATION; IDENTIFICATION; PROTEINS; EXERCISEMultiple languages
Biochemistry & Molecular Biology; BiophysicsMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/43983

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