Universität zu Köln

Brouwer, Paul, Braeutigam, Andrea, Kuelahoglu, Canan, Tazelaar, Anne O. E., Kurz, Samantha, Nierop, Klaas G. J., van der Werf, Adrie, Weber, Andreas P. M. and Schluepmann, Henriette ORCID: 0000-0001-6171-3029 (2014). Azolla domestication towards a biobased economy? New Phytol., 202 (3). S. 1069 - 1083. HOBOKEN: WILEY. ISSN 1469-8137

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Abstract

Due to its phenomenal growth requiring neither nitrogen fertilizer nor arable land and its biomass composition, the mosquito fern Azolla is a candidate crop to yield food, fuels and chemicals sustainably. To advance Azolla domestication, we research its dissemination, storage and transcriptome. Methods for dissemination, cross-fertilization and cryopreservation of the symbiosis Azolla filiculoides-Nostoc azollae are tested based on the fern spores. To study molecular processes in Azolla including spore induction, a database of 37649 unigenes from RNAseq of microsporocarps, megasporocarps and sporophytes was assembled, then validated. Spores obtained year-round germinated in vitro within 26d. In vitro fertilization rates reached 25%. Cryopreservation permitted storage for at least 7months. The unigene database entirely covered central metabolism and to a large degree covered cellular processes and regulatory networks. Analysis of genes engaged in transition to sexual reproduction revealed a FLOWERING LOCUS T-like protein in ferns with special features induced in sporulating Azolla fronds. Although domestication of a fern-cyanobacteria symbiosis may seem a daunting task, we conclude that the time is ripe and that results generated will serve to more widely access biochemicals in fern biomass for a biobased economy.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Brouwer, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED Braeutigam, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuelahoglu, Canan UNSPECIFIED UNSPECIFIED UNSPECIFIED Tazelaar, Anne O. E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kurz, Samantha UNSPECIFIED UNSPECIFIED UNSPECIFIED Nierop, Klaas G. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Werf, Adrie UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schluepmann, Henriette UNSPECIFIED orcid.org/0000-0001-6171-3029 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-440254
DOI:	10.1111/nph.12708
Journal or Publication Title:	New Phytol.
Volume:	202
Number:	3
Page Range:	S. 1069 - 1083
Date:	2014
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-8137
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ANABAENA SYMBIOSIS; FERN; C-4; FILICULOIDES; EVOLUTION; SPORES; GENES; CRYOPRESERVATION; PHOTOSYNTHESIS; TRANSCRIPTOME Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44025