Universität zu Köln

Schmitz, Jessica, Heinrichs, Luisa, Scossa, Federico ORCID: 0000-0002-6233-1679, Fernie, Alisdair R., Oelze, Marie-Luise, Dietz, Karl-Josef ORCID: 0000-0003-0311-2182, Rothbart, Maxi, Grimm, Bernhard, Fluegge, Ulf-Ingo and Haeusler, Rainer E. (2014). The essential role of sugar metabolism in the acclimation response of Arabidopsis thaliana to high light intensities. J. Exp. Bot., 65 (6). S. 1619 - 1637. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2431

Full text not available from this repository.

Abstract

Analyses of mutants impaired in assimilate export from chloroplasts revealed that carbohydrates as primary output of photosynthesis control expression of nuclear genes associated with plastidial processes such as acclimation to high light intensities.Retrograde signals from chloroplasts are thought to control the expression of nuclear genes associated with plastidial processes such as acclimation to varying light conditions. Arabidopsis mutants altered in the day and night path of photoassimilate export from the chloroplasts served as tools to study the involvement of carbohydrates in high light (HL) acclimation. A double mutant impaired in the triose phosphate/phosphate translocator (TPT) and ADP-glucose pyrophosphorylase (AGPase) (adg1-1/tpt-2) exhibits a HL-dependent depletion in endogenous carbohydrates combined with a severe growth and photosynthesis phenotype. The acclimation response of mutant and wild-type plants has been assessed in time series after transfer from low light (LL) to HL by analysing photosynthetic performance, carbohydrates, MgProtoIX (a chlorophyll precursor), and the ascorbate/glutathione redox system, combined with microarray-based transcriptomic and GC-MS-based metabolomic approaches. The data indicate that the accumulation of soluble carbohydrates (predominantly glucose) acts as a short-term response to HL exposure in both mutant and wild-type plants. Only if carbohydrates are depleted in the long term (e.g. after 2 d) is the acclimation response impaired, as observed in the adg1-1/tpt-2 double mutant. Furthermore, meta-analyses conducted with in-house and publicly available microarray data suggest that, in the long term, reactive oxygen species such as H2O2 can replace carbohydrates as signals. Moreover, a cross-talk exists between genes associated with the regulation of starch and lipid metabolism. The involvement of genes responding to phytohormones in HL acclimation appears to be less likely. Various candidate genes involved in retrograde control of nuclear gene expression emerged from the analyses of global gene expression.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schmitz, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Heinrichs, Luisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Scossa, Federico UNSPECIFIED orcid.org/0000-0002-6233-1679 UNSPECIFIED Fernie, Alisdair R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Oelze, Marie-Luise UNSPECIFIED UNSPECIFIED UNSPECIFIED Dietz, Karl-Josef UNSPECIFIED orcid.org/0000-0003-0311-2182 UNSPECIFIED Rothbart, Maxi UNSPECIFIED UNSPECIFIED UNSPECIFIED Grimm, Bernhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Fluegge, Ulf-Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED Haeusler, Rainer E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-441550
DOI:	10.1093/jxb/eru027
Journal or Publication Title:	J. Exp. Bot.
Volume:	65
Number:	6
Page Range:	S. 1619 - 1637
Date:	2014
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2431
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRIOSE-PHOSPHATE/PHOSPHATE TRANSLOCATOR; MG-PROTOPORPHYRIN IX; TRANSCRIPTION FACTORS; GENE-EXPRESSION; SINGLET OXYGEN; PHOTOSYNTHETIC ACCLIMATION; ANTHOCYANIN BIOSYNTHESIS; SIGNAL-TRANSDUCTION; OXIDATIVE STRESS; REDOX REGULATION Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44155