Universität zu Köln

Niehoff, Anja ORCID: 0000-0002-4165-0929, Lechner, Philipp, Ratiu, Oana, Reuter, Sven, Hamann, Nina, Brueggemann, Gert-Peter, Schoenau, Eckhard, Bloch, Wilhelm and Beccard, Ralf (2014). Effect of Whole-Body Vibration and Insulin-Like Growth Factor-I on Muscle Paralysis-Induced Bone Degeneration after Botulinum Toxin Injection in Mice. Calcif. Tissue Int., 94 (4). S. 373 - 384. NEW YORK: SPRINGER. ISSN 1432-0827

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Abstract

Botulinum toxin A (BTX)-induced muscle paralysis results in pronounced bone degradation with substantial bone loss. We hypothesized that whole-body vibration (WBV) and insulin-like growth factor-I (IGF-I) treatment can counteract paralysis-induced bone degradation following BTX injections by activation of the protein kinase B (Akt) signaling pathway. Female C57BL/6 mice (n = 60, 16 weeks) were assigned into six groups (n = 10 each): SHAM, BTX, BTX+WBV, BTX+IGF-I, BTX+WBV+IGF-I, and a baseline group, which was killed at the beginning of the study. Mice received a BTX (1.0 U/0.1 mL) or saline (SHAM) injection in the right hind limb. The BTX+IGF-I and BTX+WBV+IGF-I groups obtained daily subcutaneous injections of human IGF-I (1 mu g/day). The BTX+WBV and BTX+WBV+IGF-I groups underwent WBV (25 Hz, 2.1 g, 0.83 mm) for 30 min/day, 5 days/week for 4 weeks. Femora were scanned by pQCT, and mechanical properties were determined. On tibial sections TRAP staining, static histomorphometry, and immunohistochemical staining against Akt, phospho-Akt, IGF-IR (IGF-I receptor), and phospho-IGF-IR were conducted. BTX injection decreased trabecular and cortical bone mineral density. The WBV and WBV+IGF-I groups showed no difference in trabecular bone mineral density compared to the SHAM group. The phospho-IGF-IR and phospho-Akt stainings were not differentially altered in the injected hind limbs between groups. We found that high-frequency, low-magnitude WBV can counteract paralysis-induced bone loss following BTX injections, while we could not detect any effect of treatment with IGF-I.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Niehoff, Anja UNSPECIFIED orcid.org/0000-0002-4165-0929 UNSPECIFIED Lechner, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Ratiu, Oana UNSPECIFIED UNSPECIFIED UNSPECIFIED Reuter, Sven UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamann, Nina UNSPECIFIED UNSPECIFIED UNSPECIFIED Brueggemann, Gert-Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Schoenau, Eckhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Bloch, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Beccard, Ralf UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-441771
DOI:	10.1007/s00223-013-9818-3
Journal or Publication Title:	Calcif. Tissue Int.
Volume:	94
Number:	4
Page Range:	S. 373 - 384
Date:	2014
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-0827
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language B C-AKT; LOW-MAGNITUDE; LOW-LEVEL; IGF-I; OVARIECTOMIZED RATS; MECHANICAL STIMULI; WEIGHT-BEARING; DEGRADES BONE; OSTEOPOROSIS; RESISTANCE Multiple languages Endocrinology & Metabolism Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44177