Universität zu Köln

Kamolvit, Witchuda, Higgins, Paul G. ORCID: 0000-0001-8677-9454, Paterson, David L. and Seifert, Harald (2014). Multiplex PCR to detect the genes encoding naturally occurring oxacillinases in Acinetobacter spp. J. Antimicrob. Chemother., 69 (4). S. 959 - 964. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2091

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Abstract

Bacteria of the genus Acinetobacter are increasingly being isolated in hospitals and are recognized as emerging nosocomial pathogens. Species identification is difficult and there is a need for simple molecular methods to differentiate between the species. Naturally occurring oxacillinase genes (bla(OXA)) have been identified in several Acinetobacter species and their detection by PCR can aid in species identification. The aim of this study was to develop a multiplex PCR to identify intrinsic bla(OXA) genes (i.e. bla(OXA-134-like), bla(OXA-211-like), bla(OXA-213-like), bla(OXA-214-like) and bla(OXA-228-like)) from Acinetobacter spp. for use as a tool for rapid species identification. Primers were designed to selectively amplify internal fragments of intrinsic bla(OXA) from Acinetobacter lwoffii/Acinetobacter schindleri (bla(OXA-134-like)), Acinetobacter johnsonii (bla(OXA-211-like)), Acinetobacter calcoaceticus (bla(OXA-213-like)), Acinetobacter haemolyticus (bla(OXA-214-like)) and Acinetobacter bereziniae (bla(OXA-228-like)). Multiplex PCR was performed in a total of 100 Acinetobacter isolates. Flanking primers were designed for each bla(OXA) subgroup and products were sequenced. All A. lwoffii, A. schindleri, A. johnsonii, A. calcoaceticus, A. haemolyticus and A. bereziniae isolates were positive for their species-specific amplicons while other Acinetobacter species were negative. Thirty bla(OXA) novel variants were identified; the majority of these (21/30) were from A. calcoaceticus. ISAba11 was found upstream of bla(OXA-214) in four A. haemolyticus isolates, but was not associated with carbapenem resistance. This multiplex PCR specifically detected each of the five different bla(OXA) subgroups. Therefore, this method has the potential to aid in the identification of these species and monitor the spread of these genes into other Acinetobacter species.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kamolvit, Witchuda UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Paterson, David L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-442549
DOI:	10.1093/jac/dkt480
Journal or Publication Title:	J. Antimicrob. Chemother.
Volume:	69
Number:	4
Page Range:	S. 959 - 964
Date:	2014
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2091
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INSERTION-SEQUENCE ISABA11; BETA-LACTAMASE; CARBAPENEM RESISTANCE; A. BAUMANNII; IDENTIFICATION; RADIORESISTENS; DIFFERENTIATE; CALCOACETICUS; BEREZINIAE; EMERGENCE Multiple languages Infectious Diseases; Microbiology; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44254